PHACTR1 抗体 (AA 468-517)
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Quick Overview for PHACTR1 抗体 (AA 468-517) (ABIN6744441)
抗原
See all PHACTR1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 468-517
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特异性
- Human PHACTR1
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预测反应
- Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Horse, Chicken (100%).
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic peptide located between aa468-517 of human PHACTR1 (Q9C0D0, NP_112210). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch, Chicken, Xenopus (100%), Turkey (81%).
Type of Immunogen: Synthetic peptide -
亚型
- IgG
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anti-Phosphatase and Actin Regulator 1 (PHACTR1) (AA 263-387) antibody
PHACTR1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Phosphatase and Actin Regulator 1 (PHACTR1) (Middle Region) antibodyPHACTR1 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 马, 兔, 斑马鱼, 犬, Saccharomyces cerevisiae WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Distilled water
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS with 2 % sucrose
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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