IDH1 抗体 (AA 290-339)
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- 抗原 See all IDH1 抗体
- IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))
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抗原表位
- AA 290-339
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适用
- 人, 小鼠, 大鼠, 犬, Cow, 猴, 马, 豚鼠, 兔, Pig, 绵羊, Hamster, 小鸡, 非洲爪蟾
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This IDH1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human IDH1
- 预测反应
- Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken, Xenopus (100%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic peptide located between aa290-339 of human IDH1 (Q8LFC0, NP_005887). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Hamster, Dog, Cat, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Beetle (100%), Panda, Armadillo, Rice, Arabidopsis (92%), Drosophila (85%).
Type of Immunogen: Synthetic peptide - 亚型
- IgG
- Top Product
- Discover our top product IDH1 Primary Antibody
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- 应用备注
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Approved: IHC, IHC-P (5 μg/mL), WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Distilled Water.
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS with 2 % sucrose
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- 抗原
- IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))
- 别名
- IDH1 / IDH (IDH1 产品)
- 别名
- IDCD antibody, IDH antibody, IDP antibody, IDPC antibody, PICD antibody, NADP-CICDH antibody, AI314845 antibody, AI788952 antibody, E030024J03Rik antibody, Id-1 antibody, Idh-1 antibody, Idpc antibody, cb876 antibody, fm90e09 antibody, im:7143416 antibody, wu:fm90e09 antibody, F23E12.180 antibody, F23E12_180 antibody, IDH-I antibody, NAD+ DEPENDENT ISOCITRATE DEHYDROGENASE SUBUNIT 1 antibody, isocitrate dehydrogenase 1 antibody, isocitrate dehydrogenase I antibody, isocitrate dehydrogenase (NADP(+)) 1, cytosolic antibody, isocitrate dehydrogenase 1 (NADP+), soluble antibody, isocitrate dehydrogenase 1 (NADP+) L homeolog antibody, isocitrate dehydrogenase 1 antibody, IDH1 antibody, Idh1 antibody, idh1.L antibody, idh1 antibody
- 背景
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Name/Gene ID: IDH1
Synonyms: IDH1, IDCD, IDP, IDPC, PICD, NADP(+)-specific ICDH, IDH, Oxalosuccinate decarboxylase - 基因ID
- 3417
- NCBI登录号
- NP_005887
- UniProt
- O75874
- 途径
- Warburg Effect
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