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ARID5A 抗体 (C-Term)

ARID5A 适用: 人, 小鼠, 大鼠, 犬, 兔, 豚鼠, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN6741610
发货至: 中国
  • 抗原 See all ARID5A 抗体
    ARID5A (AT Rich Interactive Domain 5A (MRF1-Like) (ARID5A))
    抗原表位
    • 7
    • 6
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    C-Term
    适用
    • 25
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    人, 小鼠, 大鼠, 犬, 兔, 豚鼠, 猴
    宿主
    • 20
    • 5
    克隆类型
    • 22
    • 3
    多克隆
    标记
    • 15
    • 2
    • 2
    • 2
    • 2
    • 2
    This ARID5A antibody is un-conjugated
    应用范围
    • 24
    • 15
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    品牌
    IHC-plus™
    特异性
    Human ARID5A
    预测反应
    Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit (100%) Pig (93%) Horse (91%) Guinea pig (85%).
    纯化方法
    Immunoaffinity purified
    免疫原
    Synthetic peptide from C-Terminus of human ARID5A (Q03989, NP_997646). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Mouse, Rat, Elephant, Dog, Rabbit (100%), Bat, Pig (93%), Panda, Horse (91%), Marmoset, Guinea pig (85%), Bovine (80%).

    Type of Immunogen: Synthetic peptide
    亚型
    IgG
  • 应用备注
    Approved: IHC, IHC-P (5 μg/mL), WB

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Distilled Water.
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS with 2 % sucrose
    注意事项
    Avoid repeat freeze-thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • 抗原
    ARID5A (AT Rich Interactive Domain 5A (MRF1-Like) (ARID5A))
    别名
    MRF-1 / ARID5A (ARID5A 产品)
    别名
    MRF-1 antibody, MRF1 antibody, RP11-363D14 antibody, D430024K22Rik antibody, Mrf1 antibody, AT-rich interaction domain 5A antibody, AT rich interactive domain 5A (MRF1-like) antibody, ARID5A antibody, Arid5a antibody, arid5a antibody
    背景
    Name/Gene ID: ARID5A

    Synonyms: ARID5A, Modulator recognition factor 1, MRF-1, MRF1, RFVG5814, RP11-363D14, Modulator recognition factor I
    基因ID
    10865
    NCBI登录号
    NP_997646
    UniProt
    Q03989
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