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MED4 抗体 (AA 146-195)

MED4 适用: 人, 大鼠, 小鼠, Cow, 斑马鱼, 犬, 豚鼠, 马, 兔, 小鸡, 猴, 非洲爪蟾, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN6741609
发货至: 中国
  • 抗原 See all MED4 抗体
    MED4 (Mediator Complex Subunit 4 (MED4))
    抗原表位
    • 15
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 146-195
    适用
    人, 大鼠, 小鼠, Cow, 斑马鱼, 犬, 豚鼠, 马, 兔, 小鸡, 猴, 非洲爪蟾, Pig
    宿主
    • 31
    • 2
    克隆类型
    • 30
    • 3
    多克隆
    标记
    • 15
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This MED4 antibody is un-conjugated
    应用范围
    • 28
    • 13
    • 13
    • 13
    • 6
    • 3
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    品牌
    IHC-plus™
    特异性
    Human MED4
    预测反应
    Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Rabbit, Guinea pig, Chicken, Xenopus, Zebrafish (100%).
    纯化方法
    Immunoaffinity purified
    免疫原
    Synthetic peptide located between aa146-195 of human MED4 (Q9NPJ6, NP_054885). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Catfish, Salmon, Stickleback, Pike, Zebrafish (100%), Drosophila, Mosquito (83%).

    Type of Immunogen: Synthetic peptide
    亚型
    IgG
    Top Product
    Discover our top product MED4 Primary Antibody
  • 应用备注
    Approved: IHC, IHC-P (5 μg/mL), WB

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Distilled Water.
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS with 2 % sucrose
    注意事项
    Avoid repeat freeze-thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • 抗原
    MED4 (Mediator Complex Subunit 4 (MED4))
    别名
    MED4 (MED4 产品)
    别名
    zgc:110646 antibody, ARC36 antibody, DRIP36 antibody, HSPC126 antibody, TRAP36 antibody, VDRIP antibody, Vdrip antibody, 2410046H15Rik antibody, Vdirp antibody, mediator complex subunit 4 antibody, mediator complex subunit 4 S homeolog antibody, med4 antibody, med4.S antibody, MED4 antibody, Med4 antibody
    背景
    Name/Gene ID: MED4

    Synonyms: MED4, ARC36, DRIP36, HSPC126, Mediator complex subunit 4, TRAP/SMCC/PC2 subunit p36, TRAP36, VDRIP
    基因ID
    29079
    NCBI登录号
    NP_054885
    UniProt
    Q9NPJ6
    途径
    Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Regulation of Lipid Metabolism by PPARalpha
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