MED4 抗体 (AA 146-195)
Quick Overview for MED4 抗体 (AA 146-195) (ABIN6741609)
抗原
See all MED4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 146-195
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品牌
- IHC-plus™
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特异性
- Human MED4
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预测反应
- Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Rabbit, Guinea pig, Chicken, Xenopus, Zebrafish (100%).
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic peptide located between aa146-195 of human MED4 (Q9NPJ6, NP_054885). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Catfish, Salmon, Stickleback, Pike, Zebrafish (100%), Drosophila, Mosquito (83%).
Type of Immunogen: Synthetic peptide -
亚型
- IgG
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anti-Mediator Complex Subunit 4 (MED4) (AA 27-140) antibody
MED4 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 4 (MED4) (AA 1-270) antibodyMED4 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2B10 unconjugated
anti-Mediator Complex Subunit 4 (MED4) antibodyMED4 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Mediator Complex Subunit 4 (MED4) (C-Term) antibodyMED4 适用: 人, 大鼠, 小鼠, Cow, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 4 (MED4) (Middle Region) antibodyMED4 适用: 人, 大鼠, 小鼠, Cow, 斑马鱼, 犬, 豚鼠, 马, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 4 (MED4) (Middle Region) antibodyMED4 适用: 人, 大鼠, 小鼠, Cow, 斑马鱼, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 4 (MED4) (C-Term) antibodyMED4 适用: 人, 大鼠, 小鼠, Cow, 斑马鱼, 犬, 豚鼠, 马, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 4 (MED4) (AA 101-200) antibodyMED4 适用: 人, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 4 (MED4) (AA 167-270) antibodyMED4 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 4 (MED4) (C-Term) antibodyMED4 适用: 人, 大鼠, 小鼠, Cow, 斑马鱼, 犬, 豚鼠, 马, 兔, 小鸡, 猴 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: IHC, IHC-P (5 μg/mL), WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Distilled Water.
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS with 2 % sucrose
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- MED4 (Mediator Complex Subunit 4 (MED4))
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别名
- MED4
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背景
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Name/Gene ID: MED4
Synonyms: MED4, ARC36, DRIP36, HSPC126, Mediator complex subunit 4, TRAP/SMCC/PC2 subunit p36, TRAP36, VDRIP -
基因ID
- 29079
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NCBI登录号
- NP_054885
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UniProt
- Q9NPJ6
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途径
- Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Regulation of Lipid Metabolism by PPARalpha
抗原
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