PNPT1 抗体 (AA 489-538)
Quick Overview for PNPT1 抗体 (AA 489-538) (ABIN6741592)
抗原
See all PNPT1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 489-538
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品牌
- IHC-plus™
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特异性
- Human PNPT1
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预测反应
- Percent identity by BLAST analysis: Human (100%) Mouse (92%) Rat, Guinea pig (85%).
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic peptide located between aa489-538 of human PNPT1 (Q8TCS8, NP_149100). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%), Galago, Marmoset, Mouse (92%), Rat, Guinea pig (85%).
Type of Immunogen: Synthetic peptide -
亚型
- IgG
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anti-Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) (AA 1-783) antibody
PNPT1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) (AA 1-783) antibodyPNPT1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) antibodyPNPT1 适用: 人 WB, IP, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) (N-Term) antibodyPNPT1 适用: 人 WB, IP, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) (Middle Region) antibodyPNPT1 适用: 人, 小鼠, 大鼠, 犬, 马, Cow, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) antibodyPNPT1 适用: 人, 小鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) (AA 551-650) antibodyPNPT1 适用: 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) (C-Term) antibodyPNPT1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) (C-Term) antibodyPNPT1 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) (C-Term) antibodyPNPT1 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: IHC, IHC-P (5 μg/mL), WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 0.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute with 50 μL sterile ddH2O.
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS with 2 % sucrose
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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