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SSX4 抗体 (AA 108-157)

SSX4 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN462200
发货至: 中国
  • 抗原 See all SSX4 抗体
    SSX4 (Synovial Sarcoma, X Breakpoint 4 (SSX4))
    抗原表位
    • 12
    • 12
    • 7
    • 7
    • 7
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 108-157
    适用
    • 43
    • 1
    • 1
    宿主
    • 39
    • 4
    克隆类型
    • 41
    • 2
    多克隆
    标记
    • 16
    • 5
    • 4
    • 4
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This SSX4 antibody is un-conjugated
    应用范围
    • 35
    • 28
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    品牌
    IHC-plus™
    特异性
    Human SSX4
    预测反应
    Percent identity by BLAST analysis: Human (100%).
    纯化方法
    Immunoaffinity purified
    免疫原
    Synthetic peptide located between aa108-157 of human SSX4 (O60224, NP_005627). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%), Monkey (85%), Gibbon (80%).

    Type of Immunogen: Synthetic peptide
    亚型
    IgG
    Top Product
    Discover our top product SSX4 Primary Antibody
  • 应用备注
    Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Distilled water
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS with 2 % sucrose
    储存条件
    4 °C,-20 °C
    储存方法
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • 抗原
    SSX4 (Synovial Sarcoma, X Breakpoint 4 (SSX4))
    别名
    SSX4 (SSX4 产品)
    别名
    CT5.4 antibody, SSX family member 4 antibody, SSX4 antibody
    背景
    Name/Gene ID: SSX4

    Synonyms: SSX4, CT5.4, Cancer/testis antigen 5.4, Protein SSX4, SSX4A, SSX4B
    基因ID
    6759
    NCBI登录号
    NP_005627
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