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NCOA3 抗体 (AA 576-625)

This anti-NCOA3 antibody is a 兔 多克隆 antibody detecting NCOA3 in WB, IHC 和 IHC (p). Suitable for 人, Cow, Pig 和 猴.
产品编号 ABIN6741542
发货至: 中国

Quick Overview for NCOA3 抗体 (AA 576-625) (ABIN6741542)

抗原

See all NCOA3 抗体
NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

适用

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人, Cow, Pig, 猴

宿主

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克隆类型

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多克隆

标记

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This NCOA3 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

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    AA 576-625

    品牌

    IHC-plus™

    特异性

    Human NCOA3

    预测反应

    Percent identity by BLAST analysis: Human (100%) Horse, Pig (92%).

    纯化方法

    Immunoaffinity purified

    免疫原

    Synthetic peptide located between aa576-625 of human NCOA3 (Q0VF45, NP_006525). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Pig (100%), Horse (92%), Marmoset, Bat (84%).

    Type of Immunogen: Synthetic peptide

    亚型

    IgG
  • 应用备注

    Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Distilled water

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS with 2 % sucrose

    注意事项

    Avoid repeat freeze-thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • 抗原

    NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

    别名

    NCOA3 / SRC-3 / AIB1

    背景

    Name/Gene ID: NCOA3

    Synonyms: NCOA3, ACTR, AIB1, BHLHe42, CAGH16, CBP-interacting protein, CTG26, AIB-1, P/CIP, TNRC14, TRAM-1, Nuclear receptor coactivator 3, PCIP, RAC-3, SRC3, TNRC16, KAT13B, NCoA-3, SRC-3

    基因ID

    8202

    NCBI登录号

    NP_006525

    UniProt

    Q9Y6Q9

    途径

    Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha
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