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FHL1 抗体 (AA 51-100)

This anti-FHL1 antibody is a 兔 多克隆 antibody detecting FHL1 in WB, IHC 和 IHC (p). Suitable for 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 绵羊, 兔, Pig 和 Hamster.
产品编号 ABIN6741535
发货至: 中国

Quick Overview for FHL1 抗体 (AA 51-100) (ABIN6741535)

抗原

See all FHL1 抗体
FHL1 (Four and A Half LIM Domains 1 (FHL1))

适用

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人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 绵羊, 兔, Pig, Hamster

宿主

  • 39
  • 8
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克隆类型

  • 41
  • 9
多克隆

标记

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This FHL1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

    • 7
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    AA 51-100

    品牌

    IHC-plus™

    特异性

    Human FHL1

    预测反应

    Percent identity by BLAST analysis: Mouse, Rat, Sheep, Dog, Bovine, Rabbit, Pig, Guinea pig (100%) Horse (92%) Human, Xenopus (91%).

    纯化方法

    Immunoaffinity purified

    免疫原

    Synthetic peptide located between aa51-100 of human FHL1 (Q6IB30, NP_001440). Percent identity by BLAST analysis: Human, Chimpanzee, Galago, Mouse, Rat, Sheep, Hamster, Dog, Bovine, Rabbit, Pig, Guinea pig (100%), Horse, Platypus, Xenopus (92%), Gorilla, Orangutan, Gibbon, Monkey, Marmoset (91%), Elephant, Turkey, Zebra finch (83%).

    Type of Immunogen: Synthetic peptide

    亚型

    IgG
  • 应用备注

    Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:62500.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Distilled water

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS with 2 % sucrose

    注意事项

    Avoid repeat freeze-thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • 抗原

    FHL1 (Four and A Half LIM Domains 1 (FHL1))

    别名

    SLIM / FHL1

    背景

    Name/Gene ID: FHL1

    Synonyms: FHL1, BA535K18.1, FHL1B, FHL1A, FLH1A, Four and a half LIM domains 1, FHL-1, KYOT, Skeletal muscle LIM-protein 1, SLIM-1, Skeletal muscle LIM-protein1, SLIMMER, XMPMA, KYO-T, LIM protein SLIMMER, SLIM, SLIM1

    基因ID

    2273

    NCBI登录号

    NP_001440

    UniProt

    Q13642
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