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RASSF1 抗体 (AA 145-260)

Name: RASSF1 抗体 (AA 145-260)
SKU: ABIN673764
Availability: OutOfStock

This anti-RASSF1 antibody is a 兔多克隆 antibody detecting RASSF1 in ELISA, IF (cc), IF (p), IHC (p) 和 IHC (fro). Suitable for 人, 小鼠和大鼠. This Primary Antibody has been cited in 2+ publications.

Formalin-fixed and paraffin-embedded human oral squamous cell labeled with Anti-RASSF1A Polyclonal Antibody, Unconjugated (ABIN673764) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated used at 1:200 dilution for 40 minutes at 37°C. (RASSF1 抗体 (AA 145-260))

Formalin-fixed and paraffin embedded rat brain labeled with Anti-RASSF1A Polyclonal Antibody, Unconjugated (ABIN673764) at 1:200 followed by conjugation to the secondary antibody and DAB staining. (RASSF1 抗体 (AA 145-260))

IHC

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-RASSF1A Polyclonal Antibody, Unconjugated (ABIN673764) at 1:200 followed by conjugation to the secondary antibody and DAB staining. (RASSF1 抗体 (AA 145-260))

IHC

4 images

(2 references)

产品编号 ABIN673764

发货至: 中国

Datasheet as PDF

Quick Overview for RASSF1 抗体 (AA 145-260) (ABIN673764)

抗原

See all RASSF1 抗体

RASSF1 (Ras Association (RalGDS/AF-6) Domain Family Member 1 (RASSF1))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This RASSF1 antibody is un-conjugated

应用范围

ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

抗原表位
14

7

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3

2

2

2

2

1

1

1

1

1

1

1

1
AA 145-260

交叉反应

人, 小鼠, 大鼠

预测反应

Cow,Sheep,Horse

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human RASSF1

亚型

IgG
anti-Ras Association (RalGDS/AF-6) Domain Family Member 1 (RASSF1) (Internal Region) antibody
RASSF1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Ras Association (RalGDS/AF-6) Domain Family Member 1 (RASSF1) (AA 101-340) antibody
RASSF1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Ras Association (RalGDS/AF-6) Domain Family Member 1 (RASSF1) (AA 1-340) antibody
RASSF1 适用: 人 WB, ELISA, IF, ICC 宿主: 小鼠 Monoclonal 3F3 unconjugated

anti-Ras Association (RalGDS/AF-6) Domain Family Member 1 (RASSF1) (Center) antibody
RASSF1 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Ras Association (RalGDS/AF-6) Domain Family Member 1 (RASSF1) (AA 1-340) antibody
RASSF1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3F3 unconjugated

anti-Ras Association (RalGDS/AF-6) Domain Family Member 1 (RASSF1) (AA 1-340), (Isoform A), (N-Term) antibody
RASSF1 适用: 人 WB, IF, EIA 宿主: 小鼠 Monoclonal 3F3 unconjugated

anti-Ras Association (RalGDS/AF-6) Domain Family Member 1 (RASSF1) (AA 23-145) antibody
RASSF1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Ras Association (RalGDS/AF-6) Domain Family Member 1 (RASSF1) (AA 1-270) antibody
RASSF1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated

anti-Ras Association (RalGDS/AF-6) Domain Family Member 1 (RASSF1) (AA 201-250) antibody
RASSF1 适用: 人, 小鼠, 大鼠, Cow, 马, Pig, 犬, 绵羊, 斑马鱼, Bat, Hamster WB 宿主: 兔 Polyclonal unconjugated

anti-Ras Association (RalGDS/AF-6) Domain Family Member 1 (RASSF1) (C-Term) antibody
RASSF1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

应用备注

ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Klacz, Wierzbicki, Wronska, Rybarczyk, Stanislawowski, Slebioda, Olejniczak, Matuszewski, Kmiec: "Decreased expression of RASSF1A tumor suppressor gene is associated with worse prognosis in clear cell renal cell carcinoma." in: International journal of oncology, Vol. 48, Issue 1, pp. 55-66, (2015) (PubMed).

Liu, Ao, Zhou, Cui, Zhou, Yuan, Xiang, Cao, Liu: "CpG island hypermethylation of multiple tumor suppressor genes associated with loss of their protein expression during rat lung carcinogenesis induced by 3-methylcholanthrene and diethylnitrosamine." in: Biochemical and biophysical research communications, Vol. 402, Issue 3, pp. 507-14, (2010) (PubMed).
抗原

RASSF1 (Ras Association (RalGDS/AF-6) Domain Family Member 1 (RASSF1))

别名

RASSF1

背景

Synonyms: 123F2, RDA32, NORE2A, RASSF1A, REH3P21, Ras association domain-containing protein 1, RASSF1
Background: Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF1 stimulation. Isoform A interacts with CDC2, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage.

基因ID

11186

UniProt

Q9NS23