Recombinant Ubiquitin 抗体 (Sortase,His tag,AVI tag)
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Quick Overview for Recombinant Ubiquitin 抗体 (Sortase,His tag,AVI tag) (ABIN6731030)
抗原
See all Ubiquitin (Ub) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
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原理
- AbFlex Ubiquitin antibody (rAb)
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品牌
- AbFlex®
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产品特性
- AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.
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纯化方法
- Protein A Chromatography
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免疫原
- This antibody was raised against bovine ubiquitin.
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亚型
- IgG2a
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anti-Ubiquitin (Ub) antibody
Ub 适用: Cow WB, IHC, ICC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin (Ub) (AA 1-229) antibodyUb 适用: 人, 大鼠, 小鼠, Cow, Pig, 犬, 马, 兔 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin (Ub) (AA 1-229) antibodyUb 适用: 人, 大鼠 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal D2 unconjugated
anti-Ubiquitin (Ub) (acLys27) antibodyUb 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin (Ub) antibodyUb 适用: 人, 大鼠, 小鼠, Pig WB, IHC, IF 宿主: 小鼠 Monoclonal 5F1 unconjugated
anti-Ubiquitin (Ub) antibody (Atto 488)Ub 适用: Cow WB, IHC, ICC, IF, IP 宿主: 兔 Polyclonal Atto 488
anti-Ubiquitin (Ub) antibody (Atto 594)Ub 适用: Cow WB, IHC, ICC, IF, IP 宿主: 兔 Polyclonal Atto 594
anti-Ubiquitin (Ub) antibody (Atto 390)Ub 适用: Cow WB, IHC, ICC, IF, IP 宿主: 兔 Polyclonal Atto 390
anti-Ubiquitin (Ub) antibody (Biotin)Ub 适用: Cow WB, IHC, ICC, IF, IP 宿主: 兔 Polyclonal Biotin
anti-Ubiquitin (Ub) antibody (HRP)Ub 适用: Cow WB, IHC, ICC, IF, IP 宿主: 兔 Polyclonal HRP
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应用备注
- WB: 0.5 - 2 μg/mL DB: 2 μg/mL AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 39741.
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说明
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This recombinant antibody has been generated using defined DNA sequences to achieve high specificity and high reproducibility. The unique advantages of this antibody are its flexible labeling and purification options as it contains a sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner. Every antibody also contains a 6xHis Tag, which can be used with nickel-based purification systems, and a Biotinylation Tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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- Ubiquitin (Ub)
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别名
- Ubiquitin
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背景
- AbFlex Ubiquitin antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. Ubiquitin is an important signaling protein that is catalytically added to proteins on lysine residues to modify their function. Addition of multiple ubiquitin proteins in a string (poly-ubiquitylation) to proteins signals their destruction by the proteasome. Mono-ubiquitylation of proteins has a much different effect, more in keeping with post-translational modification of proteins by phosphorylation or acetylation. Mono-ubiquitylation is part of a number of signal transduction pathways, including those involving histone proteins and transcriptional regulation.
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途径
- Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway
抗原
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