Recombinant CHD3 抗体 (AA 865-885) (Sortase,His tag,AVI tag)
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Quick Overview for Recombinant CHD3 抗体 (AA 865-885) (Sortase,His tag,AVI tag) (ABIN6731021)
抗原
See all CHD3 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 865-885
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原理
- AbFlex CHD3 antibody (rAb)
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品牌
- AbFlex®
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产品特性
- AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.
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纯化方法
- Protein A Chromatography
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免疫原
- This antibody was raised against a synthetic peptide corresponding to amino acid residues 865-885 of Drosophila.
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亚型
- IgG2a
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anti-Chromodomain Helicase DNA Binding Protein 3 (CHD3) antibody
CHD3 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Chromodomain Helicase DNA Binding Protein 3 (CHD3) antibodyKD Validated CHD3 适用: 人 WB, ICC, FACS, IF (p) 宿主: 兔 Monoclonal 23GB1380 unconjugated Recombinant Antibody
anti-Chromodomain Helicase DNA Binding Protein 3 (CHD3) antibodyKD Validated CHD3 适用: 人 WB, ICC, FACS, IF (p) 宿主: 兔 Monoclonal 23GB1305 unconjugated Recombinant Antibody
anti-Chromodomain Helicase DNA Binding Protein 3 (CHD3) antibodyCHD3 适用: 人, 小鼠 WB, IHC, ICC, ELISA, FACS 宿主: 小鼠 Monoclonal 2G4 unconjugated
anti-Chromodomain Helicase DNA Binding Protein 3 (CHD3) (AA 1613-1820) antibodyCHD3 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Chromodomain Helicase DNA Binding Protein 3 (CHD3) (C-Term) antibodyCHD3 适用: 人, 小鼠 WB, ICC, IP 宿主: 小鼠 Monoclonal unconjugated
anti-Chromodomain Helicase DNA Binding Protein 3 (CHD3) (AA 1551-1742) antibodyCHD3 适用: 人 WB, ICC, ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Chromodomain Helicase DNA Binding Protein 3 (CHD3) (AA 1950-2000) antibodyCHD3 适用: 人, 小鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Chromodomain Helicase DNA Binding Protein 3 (CHD3) (AA 1654-1741) antibodyCHD3 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 5E12 unconjugated
anti-Chromodomain Helicase DNA Binding Protein 3 (CHD3) (AA 595-629) antibodyCHD3 适用: 人 WB 宿主: 兔 Polyclonal RB53666 unconjugated
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应用备注
- IP: 4 μg per IP WB: 0.5 - 2 μg/mL For optimal results, we recommend the addition of 0.05 % Tween 20 to all blocking solutions to reduce background. Individual optimization may be required. AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 61469.
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说明
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This recombinant antibody has been generated using defined DNA sequences to achieve high specificity and high reproducibility. The unique advantages of this antibody are its flexible labeling and purification options as it contains a sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner. Every antibody also contains a 6xHis Tag, which can be used with nickel-based purification systems, and a Biotinylation Tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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- CHD3 (Chromodomain Helicase DNA Binding Protein 3 (CHD3))
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别名
- CHD3
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背景
- AbFlex CHD3 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. CHD3 (chromodomain helicase DNA binding protein 3) is a component of the CHD family of ATP-dependent chromatin remodelers and forms histone deacetylase NuRD complexes which participate in the remodeling of chromatin by deacetylating histones.
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分子量
- 103 kDa
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NCBI登录号
- NP_649111
抗原
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