GLIS1 抗体
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北京 101111
Quick Overview for GLIS1 抗体 (ABIN7938140)
抗原
See all GLIS1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- GLIS1 antibody (mAb) (Clone 7-14)
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纯化方法
- Protein G Chromatography
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免疫原
- This antibody was raised against a peptide within the amino-terminal half of human GLIS1 (GLI-similar 1), which shares homology with mouse GLIS1.
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亚型
- IgG1 kappa
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应用备注
- WB*: 0.5 - 2 μg/mL IF: 1-2 μg/mL *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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- GLIS1 (GLIS Family Zinc Finger 1 (GLIS1))
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别名
- GLIS1
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背景
- GLIS1 (Glis Family Zinc Finger 1) belongs to a GLIS subfamily of Krüppel-like zinc-finger transcription factors characterized to regulate a set of downstream targets essential for cellular functions, including pancreatic development, b-cell maturation and maintenance, and insulin production. GLIS1 is enriched in unfertilized eggs and embryos and it can be used to promote direct reprogramming of somatic cells to induced pluripotent stem cells (iPS cells). GLIS1 may regulate the expression of numerous genes, either positively or negatively, and is implicated in mitral valve development, where dysregulation may lead to mitral valve prolapse (MVP).
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分子量
- 95 kDa
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NCBI登录号
- NP_671726
抗原
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