DYKDDDDK Tag 抗体 (DyLight 488)
(1 reference)
Quick Overview for DYKDDDDK Tag 抗体 (DyLight 488) (ABIN6720683)
抗原
适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-441-383
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Supplier
- Rockland
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原理
- DYKDDDDK Tag (Anti-FLAG®) Antibody Dylight™ 488 Conjugated
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交叉反应 （详细）
- The antibody recognizes the FLAG™ epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
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纯化方法
- This affinity purified antibody is directed against the FLAG™ epitope tag and is useful in determining its presence in over expressed proteins in various assays.
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免疫原
- This affinity purified antibody was purified from whole rabbit serum prepared by repeated immunizations with the Enterokinase Cleavage Site (ECS) peptide (D-Y-K-D-D-D-D-K) conjugated to KLH using maleimide. This antibody reacts with FLAG™ conjugated proteins.
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亚型
- IgG
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Labeling Ratio
- 4.0
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anti-DYKDDDDK Tag antibody
WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal FG4R unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IP 宿主: 小鼠 Monoclonal 5A8E5 unconjugated
anti-DYKDDDDK Tag antibody适用: 标记 WB, ELISA, IP, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 2F11G1 unconjugated
anti-DYKDDDDK Tag antibodyWB, IP, IF, IC 宿主: 小鼠 Monoclonal unconjugated
anti-DYKDDDDK Tag antibody适用: 人 WB 宿主: 兔 Polyclonal RB0493 unconjugated
anti-DYKDDDDK Tag antibody (HRP)WB, ELISA, IF 宿主: 小鼠 Monoclonal 5A8E5 HRP
anti-DYKDDDDK Tag antibodyWB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 29E4-G7 unconjugated
anti-DYKDDDDK Tag antibody (HRP)WB, ELISA, IP 宿主: 兔 Polyclonal HRP
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应用备注
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FLISA_Dilution: >1:20,000
ELISA_Dilution: 1:10,000 - 1:25,000
IF_Microscopy_Dilution: >1:5,000
Western_Blot_Dilution: 1:10,000 - 1:25,000
Other: User Optimized
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说明
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Suggested Applications: EM
Anti-FLAG DyLight 488 Conjugated Antibody has been tested by Western Blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 100 μL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS." in: eLife, Vol. 10, (2021) (PubMed).
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: "A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS." in: eLife, Vol. 10, (2021) (PubMed).
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- DYKDDDDK Tag
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背景
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Synonyms: rabbit anti DYKDDDDK DyLight™ 488 Conjugated, rabbit antibody for the detection of FLAG™ conjugated proteins DyLight™ 488 Conjugated
Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.
抗原
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