Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4) (AA 10-459) 抗体
Quick Overview for Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4) (AA 10-459) 抗体 (ABIN6719648)
抗原
See all Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 10-459
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原理
- Anti-MT-ND4 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-MT-ND4 Antibody Picoband® (ABIN6719648). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human MT-ND4 recombinant protein (Position: M10-S459).
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亚型
- IgG
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anti-Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4) antibody
MT-ND4 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4) (AA 406-459) antibodyMT-ND4 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4) (AA 350-450) antibodyMT-ND4 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. "Entrez Gene: MT-ND4 mitochondrially encoded NADH dehydrogenase 4". 2. Valdivieso AG, Marcucci F, Taminelli G, Guerrico AG, Alvarez S, Teiber ML, Dankert MA, Santa-Coloma TA (May 2007). "The expression of the mitochondrial gene MT-ND4 is downregulated in cystic fibrosis".Biochemical and Biophysical Research Communications. 356 (3): 805-9. -
说明
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Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4)
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别名
- MT-ND4
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背景
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Synonyms: NADH-ubiquinone oxidoreductase chain 4, NADH dehydrogenase subunit 4, MT-ND4, MTND4, NADH4, ND4,
Tissue Specificity: Expressed in lung, on the vascular capillary network within alveolar walls, and also at lower level in kidney.
Background: NADH-ubiquinone oxidoreductase chain 4 is a protein that in humans is encoded by the mitochondrial gene MT-ND4. MT-ND4 is a subunit of the respiratory chain Complex I that is believed to belong to the minimal assembly of core proteins required to catalyze NADH dehydrogenation and electron transfer to ubiquinone (coenzyme Q10). Initially, NADH binds to Complex I and transfers two electrons to the isoalloxazine ring of the flavin mononucleotide (FMN) prosthetic arm to form FMNH2. The electrons are transferred through a series of iron-sulfur (Fe-S) clusters in the prosthetic arm and finally to coenzyme Q10 (CoQ), which is reduced to ubiquinol (CoQH2). The flow of electrons changes the redox state of the protein, resulting in a conformational change and pK shift of the ionizable side chain, which pumps four hydrogen ions out of the mitochondrial matrix.
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分子量
- 36 kDa
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基因ID
- 4538
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UniProt
- P03905
抗原
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