NOX5 抗体 (AA 13-197)
Quick Overview for NOX5 抗体 (AA 13-197) (ABIN6719638)
抗原
See all NOX5 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 13-197
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原理
- Anti-NOX5 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-NOX5 Antibody Picoband® (ABIN6719638). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human NOX5 recombinant protein (Position: E13-F197).
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亚型
- IgG
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anti-NADPH Oxidase 5 (NOX5) (AA 456-765) antibody
NOX5 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-NADPH Oxidase 5 (NOX5) (AA 661-765) antibodyNOX5 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-NADPH Oxidase 5 (NOX5) (AA 501-600) antibodyNOX5 适用: 人 WB, ELISA, ICC, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-NADPH Oxidase 5 (NOX5) (AA 661-765) antibodyNOX5 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-NADPH Oxidase 5 (NOX5) (Internal Region) antibodyVerified NOX5 适用: 人 WB, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-NADPH Oxidase 5 (NOX5) (C-Term) antibodyNOX5 适用: 人 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-NADPH Oxidase 5 (NOX5) (C-Term) antibodyNOX5 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-NADPH Oxidase 5 (NOX5) (AA 680-709) antibodyNOX5 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
ELISA, 0.1-0.5 μg/mL
1. Banfi, B., Molnar, G., Maturana, A., Steger, K., Hegedus, B., Demaurex, N., Krause, K.-H. A Ca2+-activated NADPH oxidase in testis, spleen, and lymph nodes. J. Biol. Chem. 276: 37594-37601, 2001. 2. Kamiguti, A. S., Serrander, L., Lin, K., Harris, R. J., Cawley, J. C., Allsup, D. J., Slupsky, J. R., Krause, K.-H., Zuzel, M. Expression and activity of NOX5 in the circulating malignant B cells of hairy cell leukemia. J. Immun. 175: 8424-8430, 2005. -
说明
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Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- NOX5 (NADPH Oxidase 5 (NOX5))
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别名
- NOX5
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背景
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Synonyms: NADPH oxidase 5, NOX5
Tissue Specificity: Mainly expressed in pachytene spermatocytes of testis and in lymphocyte-rich areas of spleen and lymph nodes. Isoform v1 is expressed in spleen. Isoform v2 is expressed in testis. Also detected in ovary, placenta, pancreas, cardiac fibroblasts. Expressed in B-cells and prostate malignant cells. Isoform v1 and isoform v3 are expressed in epithelial colorectal adenocarcinoma cells. Isoform v2 and isoform v4 are expressed in endothelial cells. Isoform v1, isoform v2, isoform v3 and isoform v4 are expressed in pulmonary artery smooth muscle cells. Isoform v2 and isoform v5 are expressed in microvascular endothelial cells (at protein level).
Background: NOX5 (Nadph Oxidase 5), also known as NOX5A or NOX5B, is a protein which in humans is encoded by the NOX5 gene. The International Radiation Hybrid Mapping Consortium mapped the NOX5 gene to chromosome 15. NOX5 is a novel NADPH oxidase that generates superoxide and functions as an H+ channel in a Ca2+-dependent manner. It is found that, when heterologously expressed, NOX5 was quiescent in unstimulated cells. However, in response to elevations of the cytosolic Ca2+ concentration, it generated large amounts of superoxide. Using RT-PCR and Southern and Western blot analyses, NOX5 was identified as a flavin-containing Ca2+-dependent oxidase present in hairy leukemic cells (HC), but not normal B cells.
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分子量
- 86 kDa
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途径
- Proton Transport
抗原
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