PEF1 抗体 (AA 170-284)
Quick Overview for PEF1 抗体 (AA 170-284) (ABIN6719632)
抗原
See all PEF1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 170-284
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原理
- Anti-PEF1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-PEF1 Antibody Picoband® (ABIN6719632). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human PEF1 recombinant protein (Position: R170-L284).
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亚型
- IgG
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anti-Penta-EF-Hand Domain Containing 1 (PEF1) antibody
PEF1 适用: 人 WB, IHC (p), ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Penta-EF-Hand Domain Containing 1 (PEF1) (AA 1-284) antibodyPEF1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Penta-EF-Hand Domain Containing 1 (PEF1) antibodyPEF1 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-Penta-EF-Hand Domain Containing 1 (PEF1) (AA 121-230) antibodyPEF1 适用: 人, 小鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Penta-EF-Hand Domain Containing 1 (PEF1) (AA 180-229) antibodyPEF1 适用: 人, 小鼠, 犬, 马, Bat, 猴, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Kitaura Y, Watanabe M, Satoh H, Kawai T, Hitomi K, Maki M (Oct 1999). "Peflin, a novel member of the five-EF-hand-protein family, is similar to the apoptosis-linked gene 2 (ALG-2) protein but possesses nonapeptide repeats in the N-terminal hydrophobic region". Biochem Biophys Res Commun. 263 (1): 68-75. 2. Kitaura Y, Satoh H, Takahashi H, Shibata H, Maki M (Mar 2002). "Both ALG-2 and peflin, penta-EF-hand (PEF) proteins, are stabilized by dimerization through their fifth EF-hand regions". Arch Biochem Biophys.399 (1): 12-8. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- PEF1 (Penta-EF-Hand Domain Containing 1 (PEF1))
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别名
- PEF1
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背景
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Synonyms: Peflin, PEF protein with a long N-terminal hydrophobic domain, Penta-EF hand domain-containing protein 1, PEF1, ABP32, UNQ1845, PRO3573
Tissue Specificity: Pre-B-cells and B-cells but not terminally differentiated plasma cells.
Background: Peflin is a protein that in humans is encoded by the PEF1 gene. This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. The encoded protein has been shown to form a heterodimer with the programmed cell death 6 gene product and may modulate its function in Ca (2+) signaling. Alternative splicing results in multiple transcript variants and a pseudogene has been identified on chromosome 1.
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分子量
- 30 kDa
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基因ID
- 553115
抗原
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