PPCS 抗体
Quick Overview for PPCS 抗体 (ABIN6719631)
抗原
See all PPCS 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-PPCS Antibody Picoband®
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序列
- VEIEEKIVDN LQSRHTAFIG DRN
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-PPCS Antibody Picoband® (ABIN6719631). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human PPCS, which shares 73.9% and 78.2% amino acid (aa) sequence identity with mouse and rat PPCS, respectively.
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亚型
- IgG
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anti-Phosphopantothenoylcysteine Synthetase (PPCS) (AA 1-311) antibody
PPCS 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal AT10D11 unconjugated
anti-Phosphopantothenoylcysteine Synthetase (PPCS) (AA 60-109) antibodyPPCS 适用: 人, 小鼠, 大鼠, 马, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Phosphopantothenoylcysteine Synthetase (PPCS) (C-Term) antibodyPPCS 适用: 人, 小鼠, 大鼠, 马, Pig, 犬, 兔, Saccharomyces cerevisiae WB 宿主: 兔 Polyclonal unconjugated
anti-Phosphopantothenoylcysteine Synthetase (PPCS) (N-Term) antibodyPPCS 适用: 人, 小鼠, 大鼠, 马, Pig, 犬, Cow, 豚鼠, 绵羊, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
1. Daugherty, M., Polanuyer, B., Farrell, M., Scholle, M., Lykidis, A., de Crecy-Lagard, V., Osterman, A. Complete reconstitution of the human coenzyme A biosynthetic pathway via comparative genomics. J. Biol. Chem. 277: 21431-21439, 2002. 2. McHugh T, Laforce R, Gallagher P, Quinn S, Diggle P, Buchanan L (2006). "Natural history of the long-term cognitive, affective, and physical sequelae of a minor traumatic brain injury". Brain and Cognition. 60 (2): 209-11. 3. Bigler ED (2008). "Neuropsychology and clinical neuroscience of persistent post-concussive syndrome". Journal of the International Neuropsychological Society. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- PPCS (Phosphopantothenoylcysteine Synthetase (PPCS))
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别名
- PPCS
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背景
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Synonyms: Phosphopantothenate--cysteine ligase, Phosphopantothenoylcysteine synthetase, PPC synthetase, PPCS, COAB
Tissue Specificity: Expressed in heart, placenta, skeletal muscle kidney, pancreas, spleen, prostate, testis, ovary, ileum and colon. Expressed in lung endothelial and smooth muscle cells (at protein level).
Background: Phosphopantothenoylcysteine synthetase in humans is encoded by the PPCS gene. Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCS, one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine. By genomic sequence analysis, this PPCS gene ia mapped to chromosome 1.
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分子量
- 35 kDa
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基因ID
- 79717
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途径
- Ribonucleoside Biosynthetic Process
抗原
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