SRCIN1 抗体 (AA 189-287)
Quick Overview for SRCIN1 抗体 (AA 189-287) (ABIN6719623)
抗原
See all SRCIN1 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 189-287
-
原理
- Anti-SRCIN1 Antibody Picoband®
-
交叉反应 （详细）
- No cross-reactivity with other proteins.
-
产品特性
- Anti-SRCIN1 Antibody Picoband® (ABIN6719623). Tested in ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- E.coli-derived human SRCIN1 recombinant protein (Position: E189-E287).
-
亚型
- IgG
-
-
anti-SRC Kinase Signaling Inhibitor 1 (SRCIN1) (Internal Region) antibody
SRCIN1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-SRC Kinase Signaling Inhibitor 1 (SRCIN1) (Internal Region) antibodySRCIN1 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-SRC Kinase Signaling Inhibitor 1 (SRCIN1) (Internal Region) antibodySRCIN1 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-SRC Kinase Signaling Inhibitor 1 (SRCIN1) (Internal Region) antibodySRCIN1 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-SRC Kinase Signaling Inhibitor 1 (SRCIN1) (Center) antibodySRCIN1 适用: 人, 小鼠, 大鼠, 猴 WB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
ELISA, 0.1-0.5 μg/mL
1. Chin, L.-S., Nugent, R. D., Raynor, M. C., Vavalle, J. P., Li, L. SNIP, a novel SNAP-25-interacting protein implicated in regulated exocytosis. J. Biol. Chem. 275: 1191-1200, 2000. 2. Nagase, T., Kikuno, R., Hattori, A., Kondo, Y., Okumura, K., Ohara, O. Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 7: 347-355, 2000. -
说明
-
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
-
-
- SRCIN1 (SRC Kinase Signaling Inhibitor 1 (SRCIN1))
-
别名
- SRCIN1
-
背景
-
Synonyms: SRC kinase signaling inhibitor 1, SNAP-25-interacting protein, SNIP, p130Cas-associated protein, p140Cap, SRCIN1, KIAA1684, P140
Tissue Specificity: Expressed in some primary breast carcinomas where its presence is significantly associated with increased tumor size. Not detected in normal breast tissue.
Background: Using yeast 2-hybrid analysis, protein pull-down assays, and mutation analysis, it is showed that the first coiled-coil domain of rat Snip interacted with the N-terminal t-SNARE domain of Snap25 (600322). Biochemical studies demonstrated that Snip was tightly associated with rat brain cytoskeleton. In immunofluorescence and confocal microscopy of rat PC12 pheochromocytoma cells revealed colocalization of Snip with Snap25 in the actin cytoskeleton, particularly in filopodia, lamellipodia, and neuritic extensions, including the tips. Overexpression of Snip or its Snap25-interacting domain inhibited calcium-dependent exocytosis from PC12 cells. It is concluded that SNIP is involved in regulation of neurosecretion, perhaps via its interaction with SNAP25 and the cytoskeleton.
-
分子量
- 140 kDa
-
基因ID
- 80725
-
UniProt
- Q9C0H9
抗原
-
