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SRCIN1 抗体 (AA 189-287)

SRCIN1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN6719623
发货至: 中国
  • 抗原 See all SRCIN1 抗体
    SRCIN1 (SRC Kinase Signaling Inhibitor 1 (SRCIN1))
    抗原表位
    • 4
    • 1
    • 1
    • 1
    AA 189-287
    适用
    • 14
    • 5
    • 5
    • 1
    人, 小鼠, 大鼠
    宿主
    • 14
    克隆类型
    • 14
    多克隆
    标记
    • 7
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This SRCIN1 antibody is un-conjugated
    应用范围
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
    原理
    Anti-SRCIN1 Antibody Picoband®
    交叉反应 (详细)
    No cross-reactivity with other proteins.
    产品特性
    Anti-SRCIN1 Antibody Picoband® (ABIN6719623). Tested in ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    纯化方法
    Immunogen affinity purified.
    免疫原
    E.coli-derived human SRCIN1 recombinant protein (Position: E189-E287).
    亚型
    IgG
    Top Product
    Discover our top product SRCIN1 Primary Antibody
  • 应用备注
    Western blot, 0.1-0.5 μg/mL
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
    Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
    Immunocytochemistry, 0.5-1 μg/mL
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells
    ELISA, 0.1-0.5 μg/mL
    1. Chin, L.-S., Nugent, R. D., Raynor, M. C., Vavalle, J. P., Li, L. SNIP, a novel SNAP-25-interacting protein implicated in regulated exocytosis. J. Biol. Chem. 275: 1191-1200, 2000. 2. Nagase, T., Kikuno, R., Hattori, A., Kondo, Y., Okumura, K., Ohara, O. Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 7: 347-355, 2000.
    说明

    Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    浓度
    500 μg/mL
    缓冲液
    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • 抗原
    SRCIN1 (SRC Kinase Signaling Inhibitor 1 (SRCIN1))
    别名
    SRCIN1 (SRCIN1 产品)
    背景

    Synonyms: SRC kinase signaling inhibitor 1, SNAP-25-interacting protein, SNIP, p130Cas-associated protein, p140Cap, SRCIN1, KIAA1684, P140

    Tissue Specificity: Expressed in some primary breast carcinomas where its presence is significantly associated with increased tumor size. Not detected in normal breast tissue.

    Background: Using yeast 2-hybrid analysis, protein pull-down assays, and mutation analysis, it is showed that the first coiled-coil domain of rat Snip interacted with the N-terminal t-SNARE domain of Snap25 (600322). Biochemical studies demonstrated that Snip was tightly associated with rat brain cytoskeleton. In immunofluorescence and confocal microscopy of rat PC12 pheochromocytoma cells revealed colocalization of Snip with Snap25 in the actin cytoskeleton, particularly in filopodia, lamellipodia, and neuritic extensions, including the tips. Overexpression of Snip or its Snap25-interacting domain inhibited calcium-dependent exocytosis from PC12 cells. It is concluded that SNIP is involved in regulation of neurosecretion, perhaps via its interaction with SNAP25 and the cytoskeleton.

    分子量
    140 kDa
    基因ID
    80725
    UniProt
    Q9C0H9
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