METTL3 抗体 (AA 423-571)
Quick Overview for METTL3 抗体 (AA 423-571) (ABIN6719604)
抗原
See all METTL3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 423-571
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原理
- Anti-METTL3 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-METTL3/METTL3 Antibody Picoband® (ABIN6719604). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human METTL3 recombinant protein (Position: Q423-D571).
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亚型
- IgG
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anti-Methyltransferase Like 3 (METTL3) antibody
METTL3 适用: 人, 大鼠, 小鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Methyltransferase Like 3 (METTL3) (AA 1-156) antibodyMETTL3 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Methyltransferase Like 3 (METTL3) (AA 1-580) antibodyMETTL3 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Methyltransferase Like 3 (METTL3) (N-Term) antibodyMETTL3 适用: 人, 大鼠, 小鼠, 犬, 兔, 马, 豚鼠, Cow, 斑马鱼, Bat, Hamster, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Methyltransferase Like 3 (METTL3) (C-Term) antibodyMETTL3 适用: 人, 大鼠, 小鼠, 犬, 兔, 马, 豚鼠, Cow, 斑马鱼, 山羊, Pig, Bat, Hamster, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Methyltransferase Like 3 (METTL3) antibodyMETTL3 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Methyltransferase Like 3 (METTL3) antibodyMETTL3 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Methyltransferase Like 3 (METTL3) (Internal Region) antibodyMETTL3 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Methyltransferase Like 3 (METTL3) (AA 206-255) antibodyMETTL3 适用: 人, 大鼠, 小鼠, 犬, 兔, 马, 豚鼠, Cow, 斑马鱼, 山羊, Pig, Bat, Hamster, 猴, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Methyltransferase Like 3 (METTL3) (AA 503-552) antibodyMETTL3 适用: 人, 大鼠, 小鼠, 犬, 兔, 马, 豚鼠, Cow, 斑马鱼, Pig, Bat, Hamster, 猴, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
ELISA, 0.1-0.5 μg/mL
1. Alarcon, C. R., Lee, H., Goodarzi, H., Halberg, N., Tavazoie, S. F. N(6)-methyladenosine marks primary microRNAs for processing. Nature 519: 482-485, 2015. 2. Barbieri, I., Tzelepis, K., Pandolfini, L., Shi, J., Millan-Zambrano, G., Robson, S. C., Aspris, D., Migliori, V., Bannister, A. J., Han, N., De Braekeleer, E., Ponstingl, H., Hendrick, A., Vakoc, C. R., Vassiliou, G. S., Kouzarides, T.Promoter-bound METTL3 maintains myeloid leukaemia by m(6)A-dependent translation control. Nature 552: 126-131, 2017. 3. Bujnicki, J. M., Feder, M., Radlinska, M., Blumenthal, R. M. Structure prediction and phylogenetic analysis of a functionally diverse family of proteins homologous to the MT-A70 subunit of the human mRNA:m6A methyltransferase. J. Molec. Evol. 55: 431-444, 2002. -
说明
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Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- METTL3 (Methyltransferase Like 3 (METTL3))
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别名
- METTL3
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背景
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Synonyms: N6-adenosine-methyltransferase catalytic subunit
Tissue Specificity: Widely expressed at low level. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
Background: N6-adenosine-methyltransferase 70 kDa subunit (METTL3) is an enzyme that in humans is encoded by theMETTL3 gene. It is mapped to 14q11.2. This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.
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分子量
- 64 kDa
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基因ID
- 56339
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UniProt
- Q86U44
抗原
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