EHD1 抗体 (AA 324-516)
Quick Overview for EHD1 抗体 (AA 324-516) (ABIN6719599)
抗原
See all EHD1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 324-516
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原理
- Anti-EHD1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-EHD1 Antibody Picoband® (ABIN6719599). Tested in ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human EHD1 recombinant protein (Position: K324-E516).
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亚型
- IgG
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anti-EH-Domain Containing 1 (EHD1) (N-Term) antibody
Verified EHD1 适用: 人, 小鼠 WB, ELISA, IHC, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-EH-Domain Containing 1 (EHD1) (AA 401-498) antibodyEHD1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-EH-Domain Containing 1 (EHD1) (C-Term) antibodyEHD1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-EH-Domain Containing 1 (EHD1) antibodyEHD1 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-EH-Domain Containing 1 (EHD1) antibodyEHD1 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-EH-Domain Containing 1 (EHD1) (C-Term) antibodyEHD1 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, 马, 兔, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-EH-Domain Containing 1 (EHD1) (AA 410-438), (C-Term) antibodyEHD1 适用: 人 WB 宿主: 兔 Polyclonal RB32073 unconjugated
anti-EH-Domain Containing 1 (EHD1) (Middle Region) antibodyEHD1 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
ELISA, 0.1-0.5 μg/mL
1. Haider, N. B., Searby, C., Galperin, E., Mintz, L., Horowitz, M., Stone, E. M., Sheffield, V. C. Evaluation and molecular characterization of EHD1, a candidate gene for Bardet-Biedl syndrome 1 (BBS1). Gene 240: 227-232, 1999. 2. Mintz, L., Galperin, E., Pasmanik-Chor, M., Tulzinsky, S., Bromberg, Y., Kozak, C. A., Joyner, A., Fein, A., Horowitz, M. EHD1--an EH-domain-containing protein with a specific expression pattern. Genomics 59: 66-76, 1999. 3. Naslavsky, N., Boehm, M., Backlund, P. S., Jr., Caplan, S. Rabenosyn-5 and EHD1 interact and sequentially regulate protein recycling to the plasma membrane. Molec. Biol. Cell 15: 2410-2422, 2004. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- EHD1 (EH-Domain Containing 1 (EHD1))
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别名
- EHD1
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背景
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Synonyms: EH domain-containing protein 1, PAST homolog 1, hPAST1, Testilin, EHD1, PAST, CDABP0131
Tissue Specificity: Highly expressed in testis.
Background: EH domain-containing protein 1, also known as testilin or PAST homolog 1 (PAST1), is a protein that in humans is encoded by the EHD1 gene, belonging to the EHD protein family. It is mapped to 11q13.1. This gene belongs to a highly conserved gene family encoding EPS15 homology (EH) domain-containing proteins. The protein-binding EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor. The EH domain has been shown to be an important motif in proteins involved in protein-protein interactions and in intracellular sorting. The protein encoded by this gene is thought to play a role in the endocytosis of IGF1 receptors. Alternatively spliced transcript variants have been found for this gene.
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分子量
- 61 kDa
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基因ID
- 10938
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UniProt
- Q9H4M9
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途径
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
抗原
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