HECTD3 抗体
Quick Overview for HECTD3 抗体 (ABIN6719595)
抗原
See all HECTD3 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-HECTD3 Antibody Picoband®
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序列
- HYASAKVCEE KLRYAAYNCV AIDTDMSPWE E
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-HECTD3 Antibody Picoband® (ABIN6719595). Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human HECTD3, identical to the related mouse and rat sequences.
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亚型
- IgG
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anti-HECT Domain Containing 3 (HECTD3) (AA 682-785) antibody
HECTD3 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-HECT Domain Containing 3 (HECTD3) (AA 1-210) antibodyHECTD3 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, Human
1. Yu J., Lan J., Zhu Y., Li X., Lai X., Xue Y., Jin C., Huang H. The E3 ubiquitin ligase HECTD3 regulates ubiquitination and degradation of Tara. Biochem. Biophys. Res. Commun. 367:805-812(2008) -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- HECTD3 (HECT Domain Containing 3 (HECTD3))
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别名
- HECTD3
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背景
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Synonyms: E3 ubiquitin-protein ligase HECTD3, HECT domain-containing protein 3, HECT-type E3 ubiquitin transferase HECTD3, HECTD3
Tissue Specificity: Expressed in adult pancreas, small intestine, stomach, spinal cord and testis. Less pronounced expression in prostate, colon mucosal lining, and ovary.
Background: The protein encoded by this gene transfers ubiquitin from an E2 ubiquitin-conjugating enzyme to targeted substrates, leading to the degradation of those substrates. This gene is mapped to 1p34.1. The encoded protein has been shown to transfer ubiquitin to TRIOBP to facilitate cell cycle progression, and to STX8.
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分子量
- 97 kDa
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基因ID
- 79654
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UniProt
- Q5T447
抗原
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