EIF6 抗体 (AA 66-210)
Quick Overview for EIF6 抗体 (AA 66-210) (ABIN6719594)
抗原
See all EIF6 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 66-210
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原理
- Anti-EIF6 Antibody Picoband® (monoclonal, 2I11)
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-EIF6 Antibody Picoband® (monoclonal, 2I11) (ABIN6719594). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human EIF6 recombinant protein (Position: N66-T210). Human EIF6 shares 99.3% amino acid (aa) sequence identity with both mouse and rat EIF6.
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亚型
- IgG2a
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anti-Eukaryotic Translation Initiation Factor 6 (EIF6) (AA 66-210) antibody
EIF6 适用: 人, 小鼠, 大鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 6 (EIF6) (AA 1-245) antibodyEIF6 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 6 (EIF6) antibodyEIF6 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 6 (EIF6) (AA 1-245) antibodyEIF6 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 6 (EIF6) (AA 66-210) antibodyEIF6 适用: 人, 小鼠, 大鼠 WB, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 6 (EIF6) (AA 146-245) antibodyEIF6 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 6 (EIF6) (C-Term) antibodyEIF6 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 6 (EIF6) (AA 82-96), (Middle Region) antibodyEIF6 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 6 (EIF6) (C-Term) antibodyEIF6 适用: 人, 小鼠, 大鼠, 兔, Cow, 犬, 豚鼠, 马, 绵羊, Saccharomyces cerevisiae, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 6 (EIF6) (N-Term) antibodyEIF6 适用: 人, 小鼠, 大鼠, 兔, 猴, 小鸡 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
1. Ceci, M., Gaviraghi, C., Gorrini, C., Sala, L. A., Offenhauser, N., Marchisio, P. C., Biffo, S. Release of eIF6 (p27-BBP) from the 60S subunit allows 80S ribosome assembly. Nature 426: 579-584, 2003. 2. Gandin, V., Miluzio, A., Barbieri, A. M., Beugnet, A., Kiyokawa, H., Marchisio, P. C., Biffo, S. Eukaryotic initiation factor 6 is rate-limiting in translation, growth and transformation. Nature 455: 684-688, 2008. 3. Sanvito, F., Arrigo, G., Zuffardi, O., Agnelli, M., Marchisio, P. C., Biffo, S. Localization of p27 beta-4 binding protein gene (ITGB4BP) to human chromosome region 20q11.2. Genomics 52: 111-112, 1998. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- EIF6 (Eukaryotic Translation Initiation Factor 6 (EIF6))
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别名
- EIF6
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背景
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Synonyms: Eukaryotic translation initiation factor 6, eIF-6, B (2)GCN homolog, B4 integrin interactor, CAB, p27 (BBP), EIF6, EIF3A, ITGB4BP, OK/SW-cl.27
Tissue Specificity: Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle.
Background: EIF6 (Eukaryotic Translation Initiation Factor 6), also called EIF3A or ITGB4BP, is a human gene. By fluorescence in situ hybridization, Sanvito et al. (1998) mapped the ITGB4BP gene to 20q11.2. Ceci et al. (2003) demonstrated that the ribosomal 60S subunit is activated by release of EIF6. In the cytoplasm, EIF6 is bound to free 60S but not to 80S subunits. Furthermore, EIF6 interacts in the cytoplasm with RACK1, a receptor for activated protein kinase C. Gandin et al. (2008) demonstrated that mammalian eIF6 is required for efficient initiation of translation in vivo. Eif6-null mouse embryos were lethal at preimplantation. Heterozygous mice had 50 % reduction of eIF6 levels in all tissues, and showed reduced mass of hepatic and adipose tissues due to a lower number of cells and to impaired G1/S cell cycle progression.
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分子量
- 26 kDa
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基因ID
- 3992
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UniProt
- P56537
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途径
- Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
抗原
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