HMGN2 抗体
Quick Overview for HMGN2 抗体 (ABIN6719576)
抗原
See all HMGN2 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-HMGN2 Antibody Picoband®
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序列
- KKGKADAGKE GNNPAENGDA KTDQAQKAEG AGDAK
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-HMGN2 Antibody Picoband® (ABIN6719576). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human HMGN2, which shares 94.3% and 88.6% amino acid (aa) sequence identity with mouse and rat HMGN2, respectively.
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亚型
- IgG
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anti-High Mobility Group Nucleosomal Binding Domain 2 (HMGN2) (AA 2-81) antibody
HMGN2 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Nucleosomal Binding Domain 2 (HMGN2) antibodyHMGN2 适用: 人, 大鼠, 小鼠 IHC, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Nucleosomal Binding Domain 2 (HMGN2) (Internal Region) antibodyHMGN2 适用: 人, 大鼠, 小鼠 IHC, ELISA, WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Nucleosomal Binding Domain 2 (HMGN2) antibodyHMGN2 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Nucleosomal Binding Domain 2 (HMGN2) (AA 1-90) antibodyHMGN2 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Nucleosomal Binding Domain 2 (HMGN2) antibodyHMGN2 适用: 人, 大鼠, 小鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Nucleosomal Binding Domain 2 (HMGN2) (AA 1-90) antibodyHMGN2 适用: 人 ELISA, WB 宿主: 小鼠 Polyclonal unconjugated
anti-High Mobility Group Nucleosomal Binding Domain 2 (HMGN2) (N-Term) antibodyHMGN2 适用: 人, 大鼠, 小鼠, 犬, Pig, 马, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Nucleosomal Binding Domain 2 (HMGN2) (N-Term) antibodyHMGN2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Nucleosomal Binding Domain 2 (HMGN2) (AA 1-90) antibodyHMGN2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
1. Porkka, K., Laakkonen, P., Hoffman, J. A., Bernasconi, M., Ruoslahti, E. A fragment of the HMGN2 protein homes to the nuclei of tumor cells and tumor endothelial cells in vivo. Proc. Nat. Acad. Sci. 99: 7444-7449, 2002. 2. West, K. L., Ito, Y., Birger, Y., Postnikov, Y., Shirakawa, H., Bustin, M.HMGN3a and HMGN3b, two protein isoforms with a tissue-specific expression pattern, expand the cellular repertoire of nucleosome-binding proteins. J. Biol. Chem. 276: 25959-25969, 2001. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- HMGN2 (High Mobility Group Nucleosomal Binding Domain 2 (HMGN2))
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别名
- HMGN2
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背景
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Synonyms: NACHT, LRR and PYD domains-containing protein 2, Nucleotide-binding site protein 1, PYRIN domain and NACHT domain-containing protein 1, PYRIN-containing APAF1-like protein 2, NLRP2, NALP2, NBS1, PAN1, PYPAF2
Tissue Specificity: Expressed at high levels in lung, placenta and thymus and at lower levels in ovary, intestine and brain.
Background: Non-histone chromosomal protein HMG-17 is a protein that in humans is encoded by the HMGN2 gene. It is mapped to 1p36.11. The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMGN1, the encoded protein may help maintain an open chromatin configuration around transcribable genes. The protein has also been found to have antimicrobial activity against bacteria, viruses and fungi.
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分子量
- 18 kDa
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基因ID
- 3151
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UniProt
- P05204
抗原
-
