GOLPH3 抗体 (AA 56-298)
Quick Overview for GOLPH3 抗体 (AA 56-298) (ABIN6719570)
抗原
See all GOLPH3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 56-298
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原理
- Anti-GOLPH3 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-GOLPH3 Antibody Picoband® (ABIN6719570). Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human GOLPH3 recombinant protein (Position: K56-K298).
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亚型
- IgG
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anti-Golgi phosphoprotein 3 (Coat-Protein) (GOLPH3) (AA 16-150) antibody
GOLPH3 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Golgi phosphoprotein 3 (Coat-Protein) (GOLPH3) (C-Term) antibodyGOLPH3 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Golgi phosphoprotein 3 (Coat-Protein) (GOLPH3) (AA 251-279), (C-Term) antibodyGOLPH3 适用: 人, 小鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB18063 unconjugated
anti-Golgi phosphoprotein 3 (Coat-Protein) (GOLPH3) (AA 1-30), (N-Term) antibodyGOLPH3 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB18062 unconjugated
anti-Golgi phosphoprotein 3 (Coat-Protein) (GOLPH3) (Internal Region) antibodyVerified GOLPH3 适用: 人, 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Golgi phosphoprotein 3 (Coat-Protein) (GOLPH3) (AA 1-298) antibodyGOLPH3 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Golgi phosphoprotein 3 (Coat-Protein) (GOLPH3) antibodyGOLPH3 适用: 人 WB 宿主: 小鼠 Monoclonal 9A1 unconjugated
anti-Golgi phosphoprotein 3 (Coat-Protein) (GOLPH3) (AA 36-298) antibodyGOLPH3 适用: 人, 小鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Golgi phosphoprotein 3 (Coat-Protein) (GOLPH3) (AA 1-298) antibodyGOLPH3 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Golgi phosphoprotein 3 (Coat-Protein) (GOLPH3) (AA 114-163) antibodyGOLPH3 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Bell, A. W., Ward, M. A., Blackstock, W. P., Freeman, H. N. M., Choudhary, J. S., Lewis, A. P., Chotai, D., Fazel, A., Gushue, J. N., Paiement, J., Palcy, S., Chevet, E., Lafreniere-Roula, M., Solari, R., Thomas, D. Y., Rowley, A., Bergeron, J. J. M. Proteomics characterization of abundant Golgi membrane proteins. J. Biol. Chem. 276: 5152-5165, 2001. 2. Ng, M. M., Dippold, H. C., Buschman, M. D., Noakes, C. J., Field, S. J.GOLPH3L antagonizes GOLPH3 to determine Golgi morphology. Molec. Biol. Cell 24: 796-808, 2013. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- GOLPH3 (Golgi phosphoprotein 3 (Coat-Protein) (GOLPH3))
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别名
- GOLPH3
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背景
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Synonyms: Golgi phosphoprotein 3, Coat protein GPP34, Mitochondrial DNA absence factor, MIDAS, GOLPH3, GPP34
Tissue Specificity: Detected in muscle fibers of patients with mitochondrial diseases, not detected in normal muscle fibers.
Background: Golgi phosphoprotein 3 (GOLPH3) is a human protein encoded by GOLPH3 gene. It is mapped to 5p13.3. The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a peripheral membrane protein of the Golgi stack and may have a regulatory role in Golgi trafficking. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined.
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分子量
- 36 kDa
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基因ID
- 64083
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UniProt
- Q9H4A6
抗原
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