Grancalcin 抗体 (AA 42-217)
Quick Overview for Grancalcin 抗体 (AA 42-217) (ABIN6719568)
抗原
See all Grancalcin (GCA) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 42-217
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原理
- Anti-Grancalcin/GCA Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Grancalcin/GCA Antibody Picoband® (ABIN6719568). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human Grancalcin/GCA recombinant protein (Position: A42-I217).
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亚型
- IgG
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anti-Grancalcin, EF-Hand Calcium Binding Protein (GCA) (AA 1-139) antibody
GCA 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Grancalcin, EF-Hand Calcium Binding Protein (GCA) (AA 1-217) antibodyGCA 适用: 人 WB, IF, ELISA 宿主: 小鼠 Monoclonal 2F5 unconjugated
anti-Grancalcin, EF-Hand Calcium Binding Protein (GCA) (AA 19-53) antibodyGCA 适用: 人 WB, IF, FACS 宿主: 兔 Polyclonal RB57950 unconjugated
anti-Grancalcin, EF-Hand Calcium Binding Protein (GCA) (AA 1-217) antibodyGCA 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Grancalcin, EF-Hand Calcium Binding Protein (GCA) (AA 1-217) antibodyGCA 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2F11 unconjugated
anti-Grancalcin, EF-Hand Calcium Binding Protein (GCA) (AA 1-217) antibodyGCA 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Grancalcin, EF-Hand Calcium Binding Protein (GCA) (AA 39-220) antibodyGCA 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Grancalcin, EF-Hand Calcium Binding Protein (GCA) (AA 7-217) antibodyGCA 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
Flow Cytometry(Fixed), 1-3 μg/1x106 cells
ELISA, 0.1-0.5 μg/mL
1. Boyhan A, Casimir CM, French JK, Teahan CG, Segal AW (Mar 1992). "Molecular cloning and characterization of grancalcin, a novel EF-hand calcium-binding protein abundant in neutrophils and monocytes". J Biol Chem. 267 (5): 2928-33. 2. Teahan CG, Totty NF, Segal AW (Oct 1992). "Isolation and characterization of grancalcin, a novel 28 kDa EF-hand calcium-binding protein from human neutrophils". Biochem J. 286. ( Pt 2): 549-54. 3. Hansen C, Tarabykina S, la Cour JM, Lollike K, Berchtold MW (Jun 2003). "The PEF family proteins sorcin and grancalcin interact in vivo and in vitro". FEBS Lett. 545 (2-3): 151-4. 4. "Entrez Gene: GCA grancalcin, EF-hand calcium binding protein". -
说明
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Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- Grancalcin (GCA) (Grancalcin, EF-Hand Calcium Binding Protein (GCA))
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别名
- GCA
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背景
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Synonyms: Grancalcin, GCA, GCL
Tissue Specificity: Detected in neutrophils and macrophages (at protein level). Highly expressed in bone marrow.
Background: Grancalcin is a protein that in humans is encoded by the GCA gene. This gene is mapped to 2q24.2. This gene product, grancalcin, is a calcium-binding protein abundant in neutrophils and macrophages. It belongs to the penta-EF-hand subfamily of proteins which includes sorcin, calpain, and ALG-2. Grancalcin localization is dependent upon calcium and magnesium. In the absence of divalent cation, grancalcin localizes to the cytosolic fraction, with magnesium alone, it partitions with the granule fraction, and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions, suggesting a role for grancalcin in granule-membrane fusion and degranulation.
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分子量
- 25 kDa
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基因ID
- 25801
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UniProt
- P28676
抗原
-
