HOOK3 抗体 (AA 14-198)
Quick Overview for HOOK3 抗体 (AA 14-198) (ABIN6719547)
抗原
See all HOOK3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 14-198
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原理
- Anti-HOOK3 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-HOOK3 Antibody Picoband® (ABIN6719547). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human HOOK3 recombinant protein (Position: E14-R198).
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亚型
- IgG
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anti-Hook Homolog 3 (HOOK3) (AA 622-718) antibody
HOOK3 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3A5 unconjugated
anti-Hook Homolog 3 (HOOK3) (AA 357-455) antibodyHOOK3 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Hook Homolog 3 (HOOK3) (AA 489-718) antibodyHOOK3 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Hook Homolog 3 (HOOK3) (AA 1-718) antibodyHOOK3 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
ELISA, 0.1-0.5 μg/mL
1. Urnavicius, L., Lau, C. K., Elshenawy, M. M., Morales-Rios, E., Motz, C., Yildiz, A., Carter, A. P. Cryo-EM shows how dynactin recruits two dyneins for faster movement. Nature 554: 202-206, 2018. 2. Walenta, J. H., Didier, A. J., Liu, X., Kramer, H. The Golgi-associated Hook3 protein is a member of a novel family of microtubule-binding proteins. J. Cell Biol. 152: 923-934, 2001. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- HOOK3 (Hook Homolog 3 (HOOK3))
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别名
- HOOK3
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背景
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Synonyms: Protein Hook homolog 3, h-hook3, Hhk3, HOOK3
Tissue Specificity: Highly expressed in chromaffin cells of the adrenal medulla (at protein level). Detected in peripheral sympathetic ganglia (at protein level). Found in some paracrine cells in stomach and duodenum (at protein level).
Background: Protein Hook homolog 3 is a protein that in humans is encoded by the HOOK3 gene. Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. The Drosophila Hook protein is a component of the endocytic compartment.
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分子量
- 83 kDa
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基因ID
- 84376
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途径
- Stem Cell Maintenance, Maintenance of Protein Location
抗原
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