CD2AP 抗体 (AA 253-337)
Quick Overview for CD2AP 抗体 (AA 253-337) (ABIN6719544)
抗原
See all CD2AP (Cd2ap) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 253-337
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原理
- Anti-CD2AP Antibody Picoband® (monoclonal, 5F8)
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-CD2AP Antibody Picoband® (monoclonal, 5F8) (ABIN6719544). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human CD2AP recombinant protein (Position: K253-K337).
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亚型
- IgG1
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anti-CD2-Associated Protein (Cd2ap) (AA 368-623) antibody
Cd2ap 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD2-Associated Protein (Cd2ap) antibodyCd2ap 适用: 人 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD2-Associated Protein (Cd2ap) (AA 333-426) antibodyCd2ap 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD2-Associated Protein (Cd2ap) (AA 561-641) antibodyCd2ap 适用: 大鼠, 小鼠 WB, ELISA, ICC, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-CD2-Associated Protein (Cd2ap) antibodyCd2ap 适用: 人, 大鼠, 小鼠 WB, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-CD2-Associated Protein (Cd2ap) (C-Term) antibodyCd2ap 适用: 人, 大鼠, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-CD2-Associated Protein (Cd2ap) (AA 149-429) antibodyCd2ap 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
1. Cochran, J. N., Rush, T., Buckingham, S. C., Roberson, E. D. The Alzheimer's disease risk factor CD2AP maintains blood-brain barrier integrity. Hum. Molec. Genet. 24: 6667-6674, 2015. 2. Lowik, M. M., Groenen, P. J. T. A., Pronk, I., Lilien, M. R., Goldschmeding, R., Dijkman, H. B., Levtchenko, E. N., Monnens, L. A., van den Heuvel, L. P. Focal segmental glomerulosclerosis in a patient homozygous for a CD2AP mutation. Kidney Int. 72: 1198-1203, 2007. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- CD2AP (Cd2ap) (CD2-Associated Protein (Cd2ap))
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别名
- CD2AP
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背景
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Synonyms: CD2-associated protein, Adapter protein CMS, Cas ligand with multiple SH3 domains, CD2AP
Tissue Specificity: Widely expressed in fetal and adult tissues.
Background: CD2-associated protein is a protein that in humans is encoded by the CD2AP gene. This gene encodes a scaffolding molecule that regulates the actin cytoskeleton. The protein ly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains. The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells. It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis. Haploinsufficiency of this gene is implicated in susceptibility to glomerular disease.
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分子量
- 80 kDa
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基因ID
- 23607
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UniProt
- Q9Y5K6
抗原
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