LONP1 抗体 (AA 137-320)
Quick Overview for LONP1 抗体 (AA 137-320) (ABIN6719543)
抗原
See all LONP1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 137-320
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原理
- Anti-LONP1/Lon Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-LONP1/Lon Antibody Picoband® (ABIN6719543). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human LONP1/Lon recombinant protein (Position: R137-A320).
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亚型
- IgG
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anti-Lon Peptidase 1, Mitochondrial (LONP1) (AA 361-460) antibody
LONP1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Lon Peptidase 1, Mitochondrial (LONP1) (AA 1-959) antibodyLONP1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Lon Peptidase 1, Mitochondrial (LONP1) (AA 120-400) antibodyLONP1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Lon Peptidase 1, Mitochondrial (LONP1) (C-Term) antibodyLONP1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lon Peptidase 1, Mitochondrial (LONP1) (AA 614-959) antibodyLONP1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lon Peptidase 1, Mitochondrial (LONP1) (AA 389-418) antibodyLONP1 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB40823 unconjugated
anti-Lon Peptidase 1, Mitochondrial (LONP1) (AA 661-761) antibodyLONP1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3B2 unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
ELISA, 0.1-0.5 μg/mL
1. Petukhova GV, Grigorenko VG, Lykov IP, Yarovoi SV, Lipkin VM, Gorbalenya AE (February 1994). "Cloning and sequence analysis of cDNA for a human homolog of eubacterial ATP-dependent Lon proteases". FEBS Letters. 340 (1-2): 25-8. 2. Pinti M, Gibellini L, Liu Y, Xu S, Lu B, Cossarizza A (December 2015). "Mitochondrial Lon protease at the crossroads of oxidative stress, ageing and cancer". Cellular and Molecular Life Sciences. 72 (24): 4807-24. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- LONP1 (Lon Peptidase 1, Mitochondrial (LONP1))
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别名
- LONP1
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背景
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Synonyms: Lon protease homolog, mitochondrial, LONHs, Lon protease-like protein, LONP, Mitochondrial ATP-dependent protease Lon, Serine protease 15, LONP1, PRSS15
Tissue Specificity: Duodenum, heart, lung and liver, but not thymus.
Background: Lon protease homolog, mitochondrial is an enzyme that in humans is encoded by the LONP1 gene. It is mapped to 19p13.3. This gene encodes a mitochondrial matrix protein that belongs to the Lon family of ATP-dependent proteases. This protein mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides in the mitochondrial matrix. It may also have a chaperone function in the assembly of inner membrane protein complexes, and participate in the regulation of mitochondrial gene expression and maintenance of the integrity of the mitochondrial genome. Decreased expression of this gene has been noted in a patient with hereditary spastic paraplegia.
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分子量
- 106 kDa
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基因ID
- 9361
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UniProt
- P36776
抗原
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