CALB1 抗体 (AA 2-261)
Quick Overview for CALB1 抗体 (AA 2-261) (ABIN6719541)
抗原
See all CALB1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 2-261
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原理
- Anti-Calbindin/CALB1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Calbindin/CALB1 Antibody Picoband® (ABIN6719541). Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human Calbindin recombinant protein (Position: A2-N261).
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亚型
- IgG
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anti-Calbindin (CALB1) antibody
CALB1 适用: 人 WB, IHC, IF 宿主: 小鼠 Monoclonal unconjugated
anti-Calbindin (CALB1) (AA 2-175) antibodyCALB1 适用: 人, 大鼠, 小鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Calbindin (CALB1) (Internal Region) antibodyVerified CALB1 适用: 人, 大鼠 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Calbindin (CALB1) (AA 7-96) antibodyCALB1 适用: 人 IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal CALB1-3333 unconjugated
anti-Calbindin (CALB1) (AA 2-175) antibodyCALB1 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Calbindin (CALB1) (AA 3-251) antibodyCALB1 适用: 人, 大鼠, 小鼠, Cow, 斑马鱼, 小鸡, 猴, Non-Human Primate WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Calbindin (CALB1) (C-Term) antibodyCALB1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Calbindin (CALB1) antibodyMicroarray verified CALB1 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB440307 unconjugated
anti-Calbindin (CALB1) (AA 1-261) antibodyCALB1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1F6 unconjugated
anti-Calbindin (CALB1) (AA 7-96) antibodyCALB1 适用: 人 IHC 宿主: 小鼠 Monoclonal MSVA-471M unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunofluorescence, 5 μg/mL, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Parmentier M., "The human calbindins: cDNA and gene cloning.",Adv. Exp. Med. Biol. 255:233-240(1989). 2. Strauss K.I., Kuznicki J., Winsky L., Kawagoe J.I., Hammer M., Jacobowitz D.M.,"The mouse calretinin gene promoter region: structural and functional components.",Brain Res. Mol. Brain Res. 49:175-187(1997). 3. Gabrielides C., McCormack A.L., Hunt D.F., Christakos S.,"Brain calbindin-D28k and an Mr 29,000 calcium binding protein in cerebellum are different but related proteins: evidence obtained from sequence analysis by tandem mass spectrometry.",Biochemistry 30:656-662(1991). -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- CALB1 (Calbindin (CALB1))
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别名
- CALB1
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背景
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Synonyms: Calbindin, Calbindin D28, D-28K, Vitamin D-dependent calcium-binding protein, avian-type, CALB1, CAB27
Tissue Specificity: Detected in skin. In the cornea, it is detected in the epithelial basement membrane, the epithelial cells, and at a lower level in stromal cells. Stratified squamous epithelia. Found in hemidesmosomes. Expressed in cornea, oral mucosa, esophagus, intestine, kidney collecting ducts, ureter, bladder, urethra and thymus but is absent in lung, blood vessels, skeletal muscle and nerves.
Background: Calbindin is a calcium-binding protein belonging to the troponin C superfamily. And it mapped to 8q21.3. Calretinin is expressed in central and peripheral nervous system and in many normal and pathological tissues. The rat and human calretinin exhibit 98 % sequence homology and 91 % homology to many other species. Two calcium binding proteins, calbindin and calretinin, have been reported to be expressed in abundance in Purkinje cells and other cell types in the cerebellum.
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分子量
- 28 kDa
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UniProt
- P05937
抗原
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