CA2 抗体 (AA 2-260)
Quick Overview for CA2 抗体 (AA 2-260) (ABIN6719533)
抗原
See all CA2 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 2-260
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原理
- Anti-CA2 Antibody Picoband® (monoclonal, 10E11)
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-CA2 Antibody Picoband® (monoclonal, 10E11) (ABIN6719533). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human CA2 recombinant protein (Position: S2-K260). Human CA2 shares 81.1% and 80.7% amino acid (aa) sequence identity with mouse and rat CA2, respectively.
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亚型
- IgG2b
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anti-Carbonic Anhydrase II (CA2) (AA 1-260) antibody
CA2 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) antibodyCA2 适用: 犬 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) (AA 1-260) antibodyCA2 适用: 小鸡 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) (AA 2-260) antibodyCA2 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) (C-Term) antibodyCA2 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) antibodyCA2 适用: 人, 大鼠, 小鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) antibodyCA2 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) (AA 2-260) antibodyCA2 适用: 人, 大鼠, 小鼠 WB, IHC, ICC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) (AA 54-83), (N-Term) antibodyCA2 适用: 人, 小鼠 WB, IF 宿主: 兔 Polyclonal RB18368 unconjugated
anti-Carbonic Anhydrase II (CA2) (AA 201-260) antibodyCA2 适用: 人, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
1.Aramaki, S., Yoshida, I., Yoshino, M., Kondo, M., Sato, Y., Noda, K., Jo, R., Okue, A., Sai, N., Yamashita, F. Carbonic anhydrase II deficiency in three unrelated Japanese patients. J. Inherit. Metab. Dis. 16: 982-990, 1993. 2.Laitala, T., Vaananen, H. K. Inhibition of bone resorption in vitro by antisense RNA and DNA molecules targeted against carbonic anhydrase II or two subunits of vacuolar H(+)-ATPase. J. Clin. Invest. 93: 2311-2318, 1994. 3.Venta, P. J., Welty, R. J., Johnson, T. M., Sly, W. S., Tashian, R. E. Carbonic anhydrase II deficiency syndrome in a Belgian family is caused by a point mutation at an invariant histidine residue (107his-to-tyr): complete structure of the normal human CA II gene. Am. J. Hum. Genet. 49: 1082-1090, 1991. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- CA2 (Carbonic Anhydrase II (CA2))
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别名
- CA2
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背景
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Synonyms: Carbonic anhydrase 2, Carbonate dehydratase II, Carbonic anhydrase C, CAC, Carbonic anhydrase II, CA-II, CA2
Tissue Specificity: Ubiquituous. Expressed in platelets.
Background: CA2 is a cytosolic enzyme with the highest activity among all known CAs. The carbonic anhydrases (ACs) form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs relatively slowly in the absence of a catalyst. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification. This gene is mapped to 8q22.
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分子量
- 28 kDa
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基因ID
- 760
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UniProt
- P00918
抗原
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