POMT2 抗体
Quick Overview for POMT2 抗体 (ABIN6719505)
抗原
See all POMT2 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-POMT2 Antibody Picoband®
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序列
- QAARAAGRDV AAEAVARSPK R
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交叉反应 (详细)
- No cross-reactivity with other proteins.
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产品特性
- Anti-POMT2 Antibody Picoband® (ABIN6719505). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human POMT2, which shares 61.9% amino acid (aa) sequence identity with both mouse and rat POMT2.
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亚型
- IgG
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
1. Biancheri, R., Falace, A., Tessa, A., Pedemonte, M., Scapolan, S., Cassandrini, D., Aiello, C., Rossi, A., Broda, P., Zara, F., Santorelli, F. M., Minetti, C., Bruno, C. POMT2 gene mutation in limb-girdle muscular dystrophy with inflammatory changes. Biochem. Biophys. Res. Commun. 363: 1033-1037, 2007. 2. Mercuri, E., D'Amico, A., Tessa, A., Berardinelli, A., Pane, M., Messina, S., van Reeuwijk, J., Bertini, E., Muntoni, F., Santorelli, F. M. POMT2 mutation in a patient with 'MEB-like' phenotype. Neuromusc. Disord. 16: 446-448, 2006. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- POMT2 (Protein-O-Mannosyltransferase 2 (POMT2))
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别名
- POMT2
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背景
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Synonyms: Protein O-mannosyl-transferase 2, Dolichyl-phosphate-mannose--protein mannosyltransferase 2, POMT2
Tissue Specificity: Highly expressed in testis, detected at low levels in most tissues.
Background: Protein O-mannosyl-transferase 2 is an enzyme that in humans is encoded by the POMT2 gene. This gene is mapped to chromosome 14q24.3. The protein encoded by this gene is an O-mannosyltransferase that requires interaction with the product of the POMT1 gene for enzymatic function. The encoded protein is found in the membrane of the endoplasmic reticulum. Defects in this gene are a cause of Walker-Warburg syndrome (WWS).
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分子量
- 84 kDa
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基因ID
- 29954
抗原
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