NOVA1 抗体 (AA 416-509)
Quick Overview for NOVA1 抗体 (AA 416-509) (ABIN6719498)
抗原
See all NOVA1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 416-509
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原理
- Anti-Nova1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Nova1 Antibody Picoband® (ABIN6719498). Tested in ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human Nova1 recombinant protein (Position: T416-V509).
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亚型
- IgG
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anti-Neuro-Oncological Ventral Antigen 1 (NOVA1) (AA 295-510) antibody
NOVA1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Neuro-Oncological Ventral Antigen 1 (NOVA1) (Internal Region) antibodyVerified NOVA1 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Neuro-Oncological Ventral Antigen 1 (NOVA1) (AA 1-507) antibodyNOVA1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3F7 unconjugated
anti-Neuro-Oncological Ventral Antigen 1 (NOVA1) (AA 6-174) antibodyNOVA1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Neuro-Oncological Ventral Antigen 1 (NOVA1) (AA 139-173) antibodyNOVA1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB50876 unconjugated
anti-Neuro-Oncological Ventral Antigen 1 (NOVA1) (AA 408-507) antibodyNOVA1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Neuro-Oncological Ventral Antigen 1 (NOVA1) antibodyMicroarray verified NOVA1 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB312777 unconjugated
anti-Neuro-Oncological Ventral Antigen 1 (NOVA1) antibodyMicroarray verified NOVA1 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB363915 unconjugated
anti-Neuro-Oncological Ventral Antigen 1 (NOVA1) antibodyMicroarray verified NOVA1 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB253729 unconjugated
anti-Neuro-Oncological Ventral Antigen 1 (NOVA1) antibodyMicroarray verified NOVA1 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB309429 unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry(Fixed), 1-3 μg/1x106 cells ELISA, 0.1-0.5 μg/mL
1. Jensen, K. B., Dredge, B. K., Stefani, G., Zhong, R., Buckanovich, R. J., Okano, H. J., Yang, Y. Y. L., Darnell, R. B. Nova-1 regulates neuron-specific alternative splicing and is essential for neuronal viability. Neuron 25: 359-371, 2000. 2. Licatalosi, D. D., Mele, A., Fak, J. J., Ule, J., Kayikci, M., Chi, S. W., Clark, T. A., Schweitzer, A. C., Blume, J. E., Wang, X., Darnell, J. C., Darnell, R. B.HITS-CLIP yields genome-wide insights into brain alternative RNA processing. Nature 456: 464-469, 2008. 3. Ule, J., Stefani, G., Mele, A., Ruggiu, M., Wang, X., Taneri, B., Gaasterland, T., Blencowe, B. J., Darnell, R. B. An RNA map predicting Nova-dependent splicing regulation. Nature 444: 580-586, 2006. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- NOVA1 (Neuro-Oncological Ventral Antigen 1 (NOVA1))
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别名
- NOVA1
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背景
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Synonyms: RNA-binding protein Nova-1, Neuro-oncological ventral antigen 1, Onconeural ventral antigen 1, Paraneoplastic Ri antigen, Ventral neuron-specific protein 1, NOVA1
Tissue Specificity: Expressed in heart, placenta, skeletal muscle, brain, pancreas, lung, liver, but not kidney.
Background: RNA-binding protein Nova-1 is a protein that in humans is encoded by the NOVA1 gene. It is mapped to 14q12. This gene encodes a neuron-specific RNA-binding protein, a member of the Nova family of paraneoplastic disease antigens, that is recognized and inhibited by paraneoplastic antibodies. These antibodies are found in the sera of patients with paraneoplastic opsoclonus-ataxia, breast cancer, and small cell lung cancer. Alternatively spliced transcripts encoding distinct isoforms have been described.
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分子量
- 52-75 kDa
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基因ID
- 4857
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UniProt
- P51513
抗原
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