BCL9L 抗体 (AA 397-666)
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北京 101111
Quick Overview for BCL9L 抗体 (AA 397-666) (ABIN6719451)
抗原
See all BCL9L 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 397-666
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原理
- Anti-BCL9L Antibody
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-BCL9L Antibody (ABIN6719451). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human BCL9L recombinant protein (Position: E397-A666).
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亚型
- IgG
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anti-B-Cell CLL/lymphoma 9-Like (BCL9L) (AA 606-751) antibody
BCL9L 适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 3B9C1 unconjugated
anti-B-Cell CLL/lymphoma 9-Like (BCL9L) (AA 606-751) antibodyBCL9L 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 1C9G2 unconjugated
anti-B-Cell CLL/lymphoma 9-Like (BCL9L) (AA 147-176), (N-Term) antibodyBCL9L 适用: 人 WB 宿主: 兔 Polyclonal RB41624 unconjugated
anti-B-Cell CLL/lymphoma 9-Like (BCL9L) (N-Term) antibodyBCL9L 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
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说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- BCL9L (B-Cell CLL/lymphoma 9-Like (BCL9L))
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别名
- BCL9L
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背景
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Synonyms: B-cell CLL/lymphoma 9-like protein, B-cell lymphoma 9-like protein, BCL9-like protein, Protein BCL9-2, BCL9L, DLNB11
Tissue Specificity: Expressed in breast, ductal and invasive ductal carcinomas of the breast, sporadic colorectal adenomas and carcinomas (at protein level). Expressed in fetal brain. Expressed in lung, amygdala, eye, prostate, pancreatic and prostate cancers, head and neck tumors and embryonal tumor.
Background: B-cell CLL/lymphoma 9 like is a protein that in humans is encoded by the BCL9L gene. It is mapped to 11q23.3. Bcl-9 (B-cell lymphoma 9, also Protein legless homolog) is a transcriptional regulator that belongs to the Bcl-9 family of proteins. It is expressed in multiple tissues and serves to recruit Pygopus to the Wnt-pathway beta -catenin-TCF complex in the nucleus. Bcl-9 and Bcl-9-2 are considered evolutionary duplicates of Legless that perform the same task with different regulation. Human Bcl-9 is 1426 amino acids (aa) in length. It contains one phosphothreonine and three phosphoserine sites that have been identified so far, two poly-Pro regions (aa 514-517 and 970-973), and one poly-Ala segment (aa 900-903). There is one potential alternate start site at Met27, and a variant isoform exists that shows a four aa substitution for aa 13911426. Over aa 1009-1328, human Bcl-9 is 96 % aa identical to mouse Bcl-9.
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分子量
- 200 kDa
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基因ID
- 283149
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途径
- Stem Cell Maintenance
抗原
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