MID1 抗体 (AA 226-278)
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- 抗原 See all MID1 抗体
- MID1 (Midline 1 (MID1))
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抗原表位
- AA 226-278
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This MID1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 原理
- Rabbit IgG polyclonal antibody for MID1 detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.
- 交叉反应 （详细）
- No cross reactivity with other proteins.
- 产品特性
- Rabbit IgG polyclonal antibody for MID1 detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.
- 纯化方法
- Immunogen affinity purified.
- 免疫原
- E.coli-derived human MID1 recombinant protein (Position: N226-Q278).
- 亚型
- IgG
- Top Product
- Discover our top product MID1 Primary Antibody
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anti-Midline 1 (MID1) (N-Term) antibody
MID1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Midline 1 (MID1) (N-Term) antibodyMID1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Midline 1 (MID1) (AA 478-667) antibodyMID1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Midline 1 (MID1) antibodyMID1 适用: 人, 小鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Midline 1 (MID1) (N-Term) antibodyMID1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Midline 1 (MID1) (AA 441-540) antibodyMID1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2C11 unconjugated
anti-Midline 1 (MID1) (AA 478-667) antibodyMID1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Midline 1 (MID1) (N-Term) antibodyMID1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Midline 1 (MID1) (AA 71-120) antibodyMID1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Midline 1 (MID1) (AA 441-540) antibodyMID1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
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- 应用备注
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Application details: Western blot|0.1-0.5 μg/mL Immunohistochemistry(Paraffin-embedded Section)|0.5-1 μg/mL Direct ELISA|0.1-0.5 μg/mL
- 说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- 抗原
- MID1 (Midline 1 (MID1))
- 别名
- MID1 (MID1 产品)
- 别名
- BBBG1 antibody, FXY antibody, GBBB1 antibody, MIDIN antibody, OGS1 antibody, OS antibody, OSX antibody, RNF59 antibody, TRIM18 antibody, XPRF antibody, ZNFXY antibody, 61B3-R antibody, DXHXS1141 antibody, Fxy antibody, Trim18 antibody, Midline1 antibody, bbbg1 antibody, fxy antibody, gbbb1 antibody, midin antibody, ogs1 antibody, osx antibody, rnf59 antibody, trim18 antibody, xprf antibody, znfxy antibody, midline 1 antibody, midline 1 L homeolog antibody, MID1 antibody, Mid1 antibody, mid1 antibody, mid1.L antibody
- 背景
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Synonyms: E3 ubiquitin-protein ligase Midline-1, Midin, Putative transcription factor XPRF, RING finger protein 59, RING finger protein Midline-1, RING-type E3 ubiquitin transferase Midline-1, Tripartite motif-containing protein 18, MID1, FXY, RNF59, TRIM18, XPRF
Background: Midline-1 is a protein found in humans that is encoded by the MID1 gene. The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Alternative promoter use, alternative splicing and alternative polyadenylation result in multiple transcript variants that have different tissue specificities.
- 基因ID
- 4281
- UniProt
- O15344
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