MID1 抗体 (AA 226-278)
Quick Overview for MID1 抗体 (AA 226-278) (ABIN6719400)
抗原
See all MID1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 226-278
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原理
- Anti-MID1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-MID1 Antibody Picoband® (ABIN6719400). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human MID1 recombinant protein (Position: N226-Q278).
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亚型
- IgG
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anti-Midline 1 (MID1) (N-Term) antibody
MID1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Midline 1 (MID1) (N-Term) antibodyMID1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Midline 1 (MID1) (AA 478-667) antibodyMID1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Midline 1 (MID1) (AA 441-540) antibodyMID1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2C11 unconjugated
anti-Midline 1 (MID1) (N-Term) antibodyMID1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Midline 1 (MID1) (N-Term) antibodyMID1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Midline 1 (MID1) (AA 71-120) antibodyMID1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Midline 1 (MID1) (AA 441-540) antibodyMID1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Midline 1 (MID1) (AA 465-514) antibodyMID1 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 斑马鱼, 猴, Pig, Bat, 小鸡, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Midline 1 (MID1) (AA 1-212) antibodyMID1 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Trockenbacher A, Suckow V, Foerster J, Winter J, Krauss S, Ropers HH, Schneider R, Schweiger S (2001). "MID1, mutated in Opitz syndrome, encodes an ubiquitin ligase that targets phosphatase 2A for degradation". Nat. Genet. 29 (3): 287-94. 2. Landry JR, Mager DL (2003). "Widely spaced alternative promoters, conserved between human and rodent, control expression of the Opitz syndrome gene MID1". Genomics. 80 (5): 499-508. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- MID1 (Midline 1 (MID1))
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别名
- MID1
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背景
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Synonyms: E3 ubiquitin-protein ligase Midline-1, Midin, Putative transcription factor XPRF, RING finger protein 59, RING finger protein Midline-1, RING-type E3 ubiquitin transferase Midline-1, Tripartite motif-containing protein 18, MID1, FXY, RNF59, TRIM18, XPRF
Tissue Specificity: In the fetus, highest expression found in kidney, followed by brain and lung. Expressed at low levels in fetal liver. In the adult, most abundant in heart, placenta and brain.
Background: Midline-1 is a protein found in humans that is encoded by the MID1 gene. The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Alternative promoter use, alternative splicing and alternative polyadenylation result in multiple transcript variants that have different tissue specificities.
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分子量
- 75 kDa
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基因ID
- 4281
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UniProt
- O15344
抗原
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