Cullin 2 抗体 (AA 389-745)
Quick Overview for Cullin 2 抗体 (AA 389-745) (ABIN6719399)
抗原
See all Cullin 2 (CUL2) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 389-745
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原理
- Anti-CUL2 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-CUL2 Antibody Picoband® (ABIN6719399). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human CUL2 recombinant protein (Position: E389-A745).
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亚型
- IgG
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anti-Cullin 2 (CUL2) (C-Term) antibody
CUL2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cullin 2 (CUL2) (C-Term) antibodyCUL2 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cullin 2 (CUL2) (AA 591-742) antibodyCUL2 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cullin 2 (CUL2) (AA 1-100) antibodyCUL2 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Cullin 2 (CUL2) (C-Term) antibodyVerified CUL2 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Cullin 2 (CUL2) antibodyCUL2 适用: 人, 小鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cullin 2 (CUL2) (C-Term) antibodyCUL2 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cullin 2 (CUL2) (AA 1-100) antibodyCUL2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4G11 unconjugated
anti-Cullin 2 (CUL2) (AA 40-310) antibodyCUL2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Cullin 2 (CUL2) (AA 264-280), (Middle Region) antibodyCUL2 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Flow Cytometry(Fixed), 1-3 μg/1x106 cells
ELISA, 0.1-0.5 μg/mL
1. Clifford, S. C., Walsh, S., Hewson, K., Green, E. K., Brinke, A., Green, P. M., Gianelli, F., Eng, C., Maher, E. R. Genomic organization and chromosomal localization of the human CUL2 gene and the role of von Hippel-Lindau tumor suppressor-binding protein (CUL2 and VBP1) mutation and loss in renal-cell carcinoma development. Genes Chromosomes Cancer 26: 20-28, 1999. 2. Lonergan, K. M., Iliopoulos, O., Ohh, M., Kamura, T., Conaway, R. C., Conaway, J. W., Kaelin, W. G., Jr.Regulation of hypoxia-inducible mRNAs by the von Hippel-Lindau tumor suppressor protein requires binding to complexes containing elongins B/C and Cul2. Molec. Cell. Biol. 18: 732-741, 1998. 3. Pause, A., Lee, S., Worrell, R. A., Chen, D. Y. T., Burgess, W. H., Linehan, W. M., Klausner, R. D. The von Hippel-Lindau tumor-suppressor gene product forms a stable complex with human CUL-2, a member of the Cdc53 family of proteins. Proc. Nat. Acad. Sci. 94: 2156-2161, 1997. -
说明
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Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- Cullin 2 (CUL2)
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别名
- CUL2
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背景
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Synonyms: Cullin-2, CUL-2, CUL2
Tissue Specificity: Expressed by CD133-expressing progenitor cells among epidermal melanocytes (at protein level). Widely expressed with specific expression in pigment cells. Highly expressed in several malignant tissues: highly expressed in clinical melanomas, with low expression in normal skin. In melanoma, marks malignant melanoma-initiating cells (MMIC), in which clinical virulence resides as a consequence of unlimited self-renewal capacity, resulting in inexorable tumor progression and metastasis. Also highly expressed in a number of leukemia cells. Expressed in basal limbal epithelium.
Background: Cullin 2 (CUL2) is a protein that in humans is encoded by the CUL2 gene. Using immunofluorescence, it is showed that CUL2 is a cytosolic protein that can be translocated to the nucleus by VHL. By fluorescence in situ hybridization, this gene is mapped to 10p11.2-p11.1, a region reported to show loss of heterozygosity (LOH) in several forms of human cancer, including non-clear cell renal cell carcinoma. CUL2 may serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme.
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分子量
- 87 kDa
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基因ID
- 8453
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UniProt
- Q13617
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途径
- M Phase, Asymmetric Protein Localization, SARS-CoV-2 Protein Interactome
抗原
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