RNF123 抗体 (AA 12-188)
Quick Overview for RNF123 抗体 (AA 12-188) (ABIN6719387)
抗原
See all RNF123 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 12-188
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原理
- Anti-RNF123 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-R Antibody Picoband® (ABIN6719387). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human RNF123 recombinant protein (Position: R12-Y188).
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亚型
- IgG
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anti-Ring Finger Protein 123 (RNF123) (AA 1216-1314) antibody
RNF123 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3F8 unconjugated
anti-Ring Finger Protein 123 (RNF123) (AA 2-52) antibodyRNF123 适用: 人, 小鼠, 大鼠 WB, IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 123 (RNF123) (N-Term) antibodyRNF123 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 123 (RNF123) (AA 1216-1314) antibodyRNF123 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Ring Finger Protein 123 (RNF123) (AA 1213-1314) antibodyRNF123 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Kamura, T., Hara, T., Matsumoto, M., Ishida, N., Okumura, F., Hatakeyama, S., Yoshida, M., Nakayama, K., Nakayama, K. I. Cytoplasmic ubiquitin ligase KPC regulates proteolysis of p27(Kip1) at G1 phase. Nature Cell Biol. 6: 1229-1235, 2004. 2. Lu, Y., Adegoke, O. A. J., Nepveu, A., Nakayama, K. I., Bedard, N., Cheng, D., Peng, J., Wing, S. S. USP19 deubiquitinating enzyme supports cell proliferation by stabilizing KPC1, a ubiquitin ligase for p27(Kip1). Molec. Cell. Biol. 29: 547-558, 2009. Note: Erratum: Molec. Cell. Biol. 29: 3241 only, 2009. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- RNF123 (Ring Finger Protein 123 (RNF123))
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别名
- RNF123
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背景
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Synonyms: E3 ubiquitin-protein ligase RNF123, Kip1 ubiquitination-promoting complex protein 1, RING finger protein 123, RING-type E3 ubiquitin transferase RNF123, RNF123, KPC1, FP1477
Tissue Specificity: Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines.
Background: E3 ubiquitin-protein ligase RNF123 is an enzyme that in humans is encoded by the RNF123 gene. It is mapped to 3p21.31. The protein encoded by this gene contains a C-terminal RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions, and an N-terminal SPRY domain. This protein displays E3 ubiquitin ligase activity toward the cyclin-dependent kinase inhibitor 1B which is also known as p27 or KIP1. Alternative splicing results in multiple transcript variants.
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分子量
- 149 kDa
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基因ID
- 63891
抗原
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