EML4 抗体 (AA 207-423)
Quick Overview for EML4 抗体 (AA 207-423) (ABIN6719384)
抗原
See all EML4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 207-423
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原理
- Anti-EML4 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-EML4 Antibody Picoband® (ABIN6719384). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human EML4 recombinant protein (Position: K207-N423).
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亚型
- IgG
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anti-Echinoderm Microtubule Associated Protein Like 4 (EML4) (AA 360-560) antibody
EML4 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Echinoderm Microtubule Associated Protein Like 4 (EML4) (AA 1-62) antibodyEML4 适用: 人 ELISA, WB, IHC (p) 宿主: 小鼠 Monoclonal 3C10 unconjugated
anti-Echinoderm Microtubule Associated Protein Like 4 (EML4) (AA 1-62) antibodyEML4 适用: 人 ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Echinoderm Microtubule Associated Protein Like 4 (EML4) antibodyEML4 适用: 人, 小鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Echinoderm Microtubule Associated Protein Like 4 (EML4) (AA 1-63) antibodyEML4 适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 3C10 unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Heidebrecht, H. J., Buck, F., Pollmann, M., Siebert, R., Parwaresch, R. Cloning and localization of C2orf2-ropp120, a previously unknown WD repeat protein. Genomics 68: 348-350, 2000. 2. Maddalo, D., Manchado, E., Concepcion, C. P., Bonetti, C., Vidigal, J. A., Han, Y.-C., Ogrodowski, P., Crippa, A., Rekhtman, N., de Stanchina, E., Lowe, S. W., Ventura, A. In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system.Nature 516: 423-427, 2014. Note: Erratum: Nature 524: 502 only, 2015. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- EML4 (Echinoderm Microtubule Associated Protein Like 4 (EML4))
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别名
- EML4
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背景
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Synonyms: Echinoderm microtubule-associated protein-like 4, EMAP-4, Restrictedly overexpressed proliferation-associated protein, Ropp 120, EML4, C2orf2, EMAPL4
Tissue Specificity: High expression in adult thyroid, lower expression in adult and fetal kidney and fetal brain. Not expressed in other tissues.
Background: Echinoderm microtubule-associated protein-like 4 is a protein that in humans is encoded by the EML4gene. This gene is a member of the echinoderm microtubule associated protein-like family, and it mapped to 2p21. The encoded WD-repeat protein may be involved in microtubule formation. Abnormal fusion of parts of this gene with portions of the anaplastic lymphoma receptor tyrosine kinase gene, which generates EML4-ALK fusion transcripts, is one of the primary mutations associated with non-small cell lung cancer. Alternative splicing of this gene results in two transcript variants.
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分子量
- 120 kDa
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基因ID
- 27436
抗原
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