TXN 抗体 (AA 2-105)
Quick Overview for TXN 抗体 (AA 2-105) (ABIN6719381)
抗原
See all TXN 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 2-105
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原理
- Anti-Thioredoxin/TRX/Txn Antibody Picoband®
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交叉反应 (详细)
- No cross-reactivity with other proteins.
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产品特性
- Anti-Thioredoxin/TRX/Txn Antibody Picoband® (ABIN6719381). Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived mouse Thioredoxin TRX recombinant protein (Position: V2-A105).
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亚型
- IgG
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Benhar, M., Forrester, M. T., Hess, D. T., Stamler, J. S. Regulated protein denitrosylation by cytosolic and mitochondrial thioredoxins. Science 320: 1050-1054, 2008. 2. Im, J.-Y., Lee, K.-W., Woo, J.-M., Junn, E., Mouradian, M. M. DJ-1 induces thioredoxin 1 expression through the Nrf2 pathway. Hum. Molec. Genet. 21: 3013-3024, 2012. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- TXN (Thioredoxin (TXN))
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别名
- Txn
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背景
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Synonyms: Thioredoxin, Trx, ATL-derived factor, ADF, Txn, Txn1
Tissue Specificity: Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder.
Background: Thioredoxin is a class of small redox proteins known to be present in all organisms. It is mapped to 9q31.3. The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene.
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分子量
- 12 kDa
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基因ID
- 22166
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UniProt
- P10639
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途径
- Carbohydrate Homeostasis, Cell RedoxHomeostasis
抗原
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