RASAL1 抗体 (AA 721-798)
Quick Overview for RASAL1 抗体 (AA 721-798) (ABIN6719367)
抗原
See all RASAL1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 721-798
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原理
- Anti-RASAL1/RASAL1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-RASAL1/RASAL1 Antibody Picoband® (ABIN6719367). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human RASAL1 recombinant protein (Position: D721-A798).
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亚型
- IgG
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anti-RAS Protein Activator Like 1 (GAP1 Like) (RASAL1) (AA 451-550) antibody
RASAL1 适用: 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-RAS Protein Activator Like 1 (GAP1 Like) (RASAL1) antibodyRASAL1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-RAS Protein Activator Like 1 (GAP1 Like) (RASAL1) (C-Term) antibodyRASAL1 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-RAS Protein Activator Like 1 (GAP1 Like) (RASAL1) (AA 1-776) antibodyRASAL1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-RAS Protein Activator Like 1 (GAP1 Like) (RASAL1) (AA 698-804) antibodyRASAL1 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-RAS Protein Activator Like 1 (GAP1 Like) (RASAL1) (C-Term) antibodyVerified RASAL1 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-RAS Protein Activator Like 1 (GAP1 Like) (RASAL1) (AA 120-340) antibodyRASAL1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Allen, M., Chu, S., Brill, S., Stotler, C., Buckler, A. Restricted tissue expression pattern of a novel human rasGAP-related gene and its murine ortholog. Gene 218: 17-25, 1998. 2. Bechtel, W., McGoohan, S., Zeisberg, E. M., Muller, G. A., Kalbacher, H., Salant, D. J., Muller, C. A., Kalluri, R., Zeisberg, M. Methylation determines fibroblast activation and fibrogenesis in the kidney. Nature Med. 16: 544-550, 2010. 3. Jin, H., Wang, X., Ying, J., Wong, A. H. Y., Cui, Y., Srivastava, G., Shen, Z.-Y., Li, E.-M., Zhang, Q., Jin, J., Kupzig, S., Chan, A. T. C., Cullen, P. J., Tao, Q. Epigenetic silencing of a Ca(2+)-regulated Ras GTPase-activating protein RASAL defines a new mechanism of Ras activation in human cancers. Proc. Nat. Acad. Sci. 104: 12353-12358, 2007. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- RASAL1 (RAS Protein Activator Like 1 (GAP1 Like) (RASAL1))
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别名
- RASAL1
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背景
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Synonyms: RasGAP-activating-like protein 1, RAS protein activator like 1
Tissue Specificity: Highly expressed in thyroid and adrenal medulla, lower expression in brain, spinal cord and trachea (PubMed:9751798). Expressed in melanocytes (PubMed:23999003).
Background: The protein encoded by this gene is member of the GAP1 family of GTPase-activating proteins. And it is mapped to 12q24.13. These proteins stimulate the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. This particular family member contains domains which are characteristic of the GAP1 subfamily of RasGAP proteins but, in contrast to the other GAP1 family members, this protein is strongly and selectively expressed in endocrine tissues. Alternatively spliced transcript variants that encode different isoforms have been described.
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分子量
- 90 kDa
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基因ID
- 8437
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UniProt
- O95294
抗原
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