RAB5 抗体 (C-Term)
Quick Overview for RAB5 抗体 (C-Term) (ABIN6719366)
抗原
See all RAB5 (RAB5A) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 179-215, C-Term
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原理
- Anti-Rab5/RAB5A Antibody Picoband® (monoclonal, 3E9)
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序列
- KKLPKNEPQN PGANSARGRG VDLTEPTQPT RNQCCSN
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Rab5/RAB5A Antibody Picoband® (monoclonal, 3E9) (ABIN6719366). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human Rab5, different from the related mouse and rat sequences by three amino acids.
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亚型
- IgG2b
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anti-RAB5A, Member RAS Oncogene Family (RAB5A) (C-Term) antibody
RAB5A 适用: 人, 小鼠, 大鼠, Cow, 猴, 犬, 绵羊, 马, 兔, 豚鼠, Pig, Hamster, 小鸡, Avian, Cat, 驴, 山羊 WB, IF, IHC (p), IHC (fro) 宿主: 山羊 Polyclonal unconjugated
anti-RAB5A, Member RAS Oncogene Family (RAB5A) (C-Term) antibodyRAB5A 适用: 人, 小鼠, 大鼠, Cow, 猴, 犬, 绵羊, 马, 兔, 豚鼠, Pig, Hamster, 小鸡, Avian, Cat, 驴, 山羊 WB, IF 宿主: 山羊 Polyclonal unconjugated
anti-RAB5A, Member RAS Oncogene Family (RAB5A) antibodyRAB5A 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-RAB5A, Member RAS Oncogene Family (RAB5A) (AA 1-215) antibodyRAB5A 适用: 人 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 3H5C11 unconjugated
anti-RAB5A, Member RAS Oncogene Family (RAB5A) (AA 1-215) antibodyRAB5A 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-RAB5A, Member RAS Oncogene Family (RAB5A) antibody (Atto 390)RAB5A 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal Atto 390
anti-RAB5A, Member RAS Oncogene Family (RAB5A) (AA 182-211) antibodyRAB5A 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-RAB5A, Member RAS Oncogene Family (RAB5A) antibody (HRP)RAB5A 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal HRP
anti-RAB5A, Member RAS Oncogene Family (RAB5A) antibody (FITC)RAB5A 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal FITC
anti-RAB5A, Member RAS Oncogene Family (RAB5A) antibody (Biotin)RAB5A 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal Biotin
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
1.Bucci, C., Parton, R. G., Mather, I. H., Stunnenberg, H., Simons, K., Hoflack, B., Zerial, M. The small GTPase rab5 functions as a regulatory factor in the early endocytic pathway. Cell 70: 715-728, 1992. 2.Kitano, M., Nakaya, M., Nakamura, T., Nagata, S., Matsuda, M. Imaging of Rab5 activity identifies essential regulators for phagosome maturation. Nature 453: 241-245, 2008. 3.Lanzetti, L., Rybin, V., Malabarba, M. G., Christoforidis, S., Scita, G., Zerial, M., Di Fiore, P. P. The Eps8 protein coordinates EGF receptor signalling through Rac and trafficking through Rab5. Nature 408: 374-377, 2000. -
说明
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Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))
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别名
- RAB5A
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背景
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Synonyms: Ras-related protein Rab-5A, RAB5A, RAB5
Tissue Specificity: Widely expressed in fetal and adult tissues.
Background: RAB5A (Ras-associated protein RAB5A), also called RAB5, is a protein that in humans is encoded by the RAB5A gene. RAB5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway. The RAB5A gene is mapped on 3p24.3. RAB5 is indispensable for a form of receptor tyrosine kinase-induced actin remodeling called circular ruffling.It signals to the actin cytoskeleton through RNTRE, a RAB5-specific GTPase-activating protein (GAP).RAB5 activity on phagosome membranes began to increase on disassembly of the actin coat encapsulating phagosomes. In addition, RAB5 activation is either continuous or repetitive for up to 10 minutes, but it ends before the collapse of engulfed apoptotic cells. Expression of a dominant-negative mutant of RAB5 delayed this collapse of apoptotic thymocytes, showing a role for RAB5 in phagosome maturation.
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分子量
- 24 kDa
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基因ID
- 5868
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UniProt
- P20339
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途径
- Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity
抗原
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