Olfactomedin 4 抗体 (AA 91-510)
Quick Overview for Olfactomedin 4 抗体 (AA 91-510) (ABIN6719350)
抗原
See all Olfactomedin 4 (OLFM4) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 91-510
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原理
- Anti-OLFM4 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-OLFM4 Antibody Picoband® (ABIN6719350). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human OLFM4 recombinant protein (Position: D91-Q510).
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亚型
- IgG
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anti-Olfactomedin 4 (OLFM4) (AA 238-500) antibody
OLFM4 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Olfactomedin 4 (OLFM4) (AA 111-510) antibodyOLFM4 适用: 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Olfactomedin 4 (OLFM4) (AA 224-334) antibodyOLFM4 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Olfactomedin 4 (OLFM4) (AA 405-511) antibodyOLFM4 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Olfactomedin 4 (OLFM4) (AA 211-510) antibodyOLFM4 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Olfactomedin 4 (OLFM4) antibodyOLFM4 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Olfactomedin 4 (OLFM4) (AA 407-510) antibodyOLFM4 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Olfactomedin 4 (OLFM4) (C-Term) antibodyOLFM4 适用: 人, 犬, Cow, 豚鼠, 马, 兔, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Olfactomedin 4 (OLFM4) (C-Term) antibodyOLFM4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Dassen H, Punyadeera C, Delvoux B, Schulkens I, Marchetti C, Kamps R, Klomp J, Dijcks F, de Goeij A, D'Hooghe T, Kyama C, Ederveen A, Dunselman G, Groothuis P, Romano A (2010). "Olfactomedin-4 regulation by estrogen in the human endometrium requires epidermal growth factor signaling". Am. J. Pathol. 177 (5): 2495-508. 2. van der Flier LG, Haegebarth A, Stange DE, van de Wetering M, Clevers H (2009). "OLFM4 is a robust marker for stem cells in human intestine and marks a subset of colorectal cancer cells".Gastroenterology. 137 (1): 15-7. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- Olfactomedin 4 (OLFM4)
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别名
- OLFM4
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背景
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Synonyms: Olfactomedin-4, OLM4, Antiapoptotic protein GW112, G-CSF-stimulated clone 1 protein, hGC-1, hOLfD, OLFM4, GW112, UNQ362, PRO698
Tissue Specificity: Expressed during myeloid lineage development. Much higher expression in bone marrow neutrophils than in peripheral blood neutrophils (at protein level). Strongly expressed in the prostate, small intestine and colon and moderately expressed in the bone marrow and stomach. Overexpressed in some pancreatic cancer tissues.
Background: Olfactomedin 4 is a protein that in humans is encoded by the OLFM4 gene. This gene was originally cloned from human myeloblasts and found to be selectively expressed in inflammed colonic epithelium. This gene encodes a member of the olfactomedin family. The encoded protein is an antiapoptotic factor that promotes tumor growth and is an extracellular matrix glycoprotein that facilitates cell adhesion.
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分子量
- 75 kDa
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基因ID
- 10562
抗原
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