MED15 抗体 (AA 1-285)
Quick Overview for MED15 抗体 (AA 1-285) (ABIN6719342)
抗原
See all MED15 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-285
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原理
- Anti-MED15 Antibody Picoband® (monoclonal, 6F4)
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-MED15 Antibody Picoband® (monoclonal, 6F4) (ABIN6719342). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human MED15 recombinant protein (Position: M1-A285).
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亚型
- IgG1
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anti-Mediator Complex Subunit 15 (MED15) (AA 1-285) antibody
MED15 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 15 (MED15) (AA 1-88) antibodyMED15 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4A4 unconjugated
anti-Mediator Complex Subunit 15 (MED15) (AA 712-746), (C-Term) antibodyMED15 适用: 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB51171 unconjugated
anti-Mediator Complex Subunit 15 (MED15) (AA 433-530) antibodyMED15 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 15 (MED15) (C-Term) antibodyMED15 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 15 (MED15) (C-Term) antibodyMED15 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 15 (MED15) antibodyMED15 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 15 (MED15) (AA 743-779), (C-Term) antibodyMED15 适用: 人, 大鼠 WB 宿主: 兔 Polyclonal RB51972 unconjugated
anti-Mediator Complex Subunit 15 (MED15) (Internal Region) antibodyVerified MED15 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
1.Fassler JS, Winston F (Dec 1989). "The Saccharomyces cerevisiae SPT13/GAL11 gene has both positive and negative regulatory roles in transcription". Molecular and Cellular Biology. 9 (12): 5602-9. 2.Suzuki Y, Nogi Y, Abe A, Fukasawa T (Nov 1988). "GAL11 protein, an auxiliary transcription activator for genes encoding galactose-metabolizing enzymes in Saccharomyces cerevisiae". Molecular and Cellular Biology. 8 (11): 4991-9. -
说明
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Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- MED15 (Mediator Complex Subunit 15 (MED15))
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别名
- MED15
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背景
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Synonyms: Mediator of RNA polymerase II transcription subunit 15, Activator-recruited cofactor 105 kDa component, ARC105, CTG repeat protein 7a, Mediator complex subunit 15, Positive cofactor 2 glutamine, Q-rich-associated protein, PC2 glutamine, Q-rich-associated protein, TPA-inducible gene 1 protein, TIG-1, Trinucleotide repeat-containing gene 7 protein, MED15, ARC105, CTG7A, PCQAP, TIG1, TNRC7
Tissue Specificity: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also found in a number of breast tumor cell lines.
Background: Mediator of RNA polymerase II transcription subunit 15, also known as Gal11, Spt13 in yeast and PCQAP, ARC105, or TIG-1 in humans is a protein encoded by the MED15 gene. The protein encoded by this gene is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. This gene contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome.
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分子量
- 86 kDa
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基因ID
- 51586
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途径
- Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha
抗原
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