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Fibronectin 抗体 (AA 1201-1300)

Name: Fibronectin 抗体 (AA 1201-1300)
SKU: ABIN671646
Availability: OutOfStock

This anti-Fibronectin antibody is a 兔多克隆 antibody detecting Fibronectin in WB, ELISA, ICC, IHC (p), IF (p), IHC (fro) 和 IF (cc). Suitable for 人, 大鼠, 小鼠和 Cow. This Primary Antibody has been cited in 13+ publications.

Image kindly submitted by Edita Aksamitiene as part of the free sample program. (Fibronectin 抗体 (AA 1201-1300))

Formalin-fixed and rat pancreas tissue labeled with Anti-FN/Fibronectin 1 Polyclonal Antibody, Unconjugated (ABIN671646) at 1:200 followed by conjugation to the secondary antibody, Goat Anti-Rabbit IgG, PE conjugated (Fibronectin 抗体 (AA 1201-1300))

Paraformaldehyde-fixed, paraffin embedded human kidney tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-Fibronectin Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining (Fibronectin 抗体 (AA 1201-1300))

IHC (p)

Image provided by One World Lab validation program. (Fibronectin 抗体 (AA 1201-1300))

IF (cc)

Paraformaldehyde-fixed, paraffin embedded rat intestine tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-Fibronectin Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining (Fibronectin 抗体 (AA 1201-1300))

IHC (p)

Downregulation of miR-27a with a miR-27a inhibitor prevented high glucose (HG)-induced mesangial cell (MC) proliferation and the expression of ECM-associated profibrotic genes via upregulation of PPARγ expression. (Fibronectin 抗体 (AA 1201-1300))

Inhibition of miR-27a with antagomir-27a reduces the expression of profibrotic genes and ECM proteins in the kidney glomeruli of STZ-induced diabetic rats. (Fibronectin 抗体 (AA 1201-1300))

IHC (p)

7 images

(13 references)

产品编号 ABIN671646

发货至: 中国

Quick Overview for Fibronectin 抗体 (AA 1201-1300) (ABIN671646)

抗原

See all Fibronectin 抗体

Fibronectin

适用

人, 大鼠, 小鼠, Cow

宿主

兔

克隆类型

多克隆

标记

This Fibronectin antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc))

抗原表位
15

14

10

4

3

3

1

1

1

1

1

1

1

1

1
AA 1201-1300

交叉反应

Cow, 人, 小鼠, 大鼠

预测反应

Dog

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from mouse Fibronectin

亚型

IgG
anti-Fibronectin (AA 2117-2236) antibody
适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Fibronectin (AA 355-558) antibody
适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Fibronectin (AA 313-607) antibody
适用: 人 WB, IHC, IP, FACS, ICC 宿主: 兔 Polyclonal unconjugated

anti-Fibronectin (AA 1998-2106) antibody
适用: 人 WB, IHC, IP, FACS, ICC 宿主: 兔 Polyclonal unconjugated

anti-Fibronectin (AA 2240-2280) antibody
适用: 人, 大鼠, 小鼠 WB, IHC (p), IF (p) 宿主: 小鼠 Monoclonal 1B9 unconjugated

anti-Fibronectin (AA 510-689) antibody
适用: 小鸡 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Fibronectin (AA 355-558) antibody
适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C2 unconjugated

anti-Fibronectin (AA 2206-2337) antibody
适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Fibronectin antibody
KD Validated 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Monoclonal unconjugated Recombinant Antibody

anti-Fibronectin (AA 1201-1300) antibody (FITC)
适用: 人, 大鼠, 小鼠, Cow WB, IF (p), IF (cc) 宿主: 兔 Polyclonal FITC

应用备注

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Miyauchi, Yasuchika, Fukumitsu, Ishii, Ogiso, Minami, Kojima, Yamaoka, Katayama, Kawai, Yoshitoshi-Uebayashi, Kita, Yasuda, Sasaki, Uemoto: "A novel three-dimensional culture system maintaining the physiological extracellular matrix of fibrotic model livers accelerates progression of hepatocellular carcinoma cells." in: Scientific reports, Vol. 7, Issue 1, pp. 9827, (2017) (PubMed).

Yang, Li, Qi, Li, Zhou, Qing, Zhang, Gao: "lncRNA H19 is involved in TGF-β1-induced epithelial to mesenchymal transition in bovine epithelial cells through PI3K/AKT Signaling Pathway." in: PeerJ, Vol. 5, pp. e3950, (2017) (PubMed).

Liu, Xiao: "Notch1 signaling induces epithelial-mesenchymal transition in lens epithelium cells during hypoxia." in: BMC ophthalmology, Vol. 17, Issue 1, pp. 135, (2017) (PubMed).

Elmas, Erbas, Yigitturk: "The efficacy of Aesculus hippocastanum seeds on diabetic nephropathy in a streptozotocin-induced diabetic rat model." in: Biomedicine & pharmacotherapy, Vol. 83, pp. 392-396, (2016) (PubMed).

Kang, Baik, Yoo, Kim, Cheong, Park, Kang, Ha: "Aliskiren Regulates Neonatal Fc Receptor and IgG Metabolism with Attenuation of Anti-GBM Glomerulonephritis in Mice." in: Nephron, Vol. 134, Issue 4, pp. 272-282, (2016) (PubMed).

Ji, Wu, Ma, Ma, Qin: "The effect of resveratrol on the expression of AdipoR1 in kidneys of diabetic nephropathy." in: Molecular biology reports, Vol. 41, Issue 4, pp. 2151-9, (2014) (PubMed).

Karadeniz, Cavuso?lu, Turkmen, Uyan?kgil, Karadeniz, Akdemir, Tuglu, Ates, Erbas: "Experimental comparison of protective characteristics of enalapril and trimetazidine in diabetic nephropathy." in: Renal failure, Vol. 36, Issue 8, pp. 1283-90, (2014) (PubMed).

Guo, Li, Dong, Chen, Deng, Wang, Ying: "Piezoelectric PU/PVDF electrospun scaffolds for wound healing applications." in: Colloids and surfaces. B, Biointerfaces, Vol. 96, pp. 29-36, (2012) (PubMed).

Wu, Zhang, Ma, Zhang, Qin: "The effect of resveratrol on FoxO1 expression in kidneys of diabetic nephropathy rats." in: Molecular biology reports, Vol. 39, Issue 9, pp. 9085-93, (2012) (PubMed).

Bai, Zheng, Fang, Wang, Hou, Li, Chen, Tian: "Fabrication of engineered heart tissue grafts from alginate/collagen barium composite microbeads." in: Biomedical materials (Bristol, England), Vol. 6, Issue 4, pp. 45002, (2011) (PubMed).

Xu, Chen, Huang, Varghese, Moorhead, Yan, Powis, Li, Ruan: "Inflammatory stress exacerbates lipid-mediated renal injury in ApoE/CD36/SRA triple knockout mice." in: American journal of physiology. Renal physiology, Vol. 301, Issue 4, pp. F713-22, (2011) (PubMed).

Ma, Tao, Lu, Jiang: "Intraocular expression of serum amyloid a and interleukin-6 in proliferative diabetic retinopathy." in: American journal of ophthalmology, Vol. 152, Issue 4, pp. 678-685.e2, (2011) (PubMed).

Jun, Song, Chen, Zanhua, Yonghong, Shuxia, Huijun: "In vivo and in vitro effects of SREBP-1 on diabetic renal tubular lipid accumulation and RNAi-mediated gene silencing study." in: Histochemistry and cell biology, Vol. 131, Issue 3, pp. 327-45, (2009) (PubMed).
抗原

Fibronectin

背景

Synonyms: Fn, Fn-1, E3327I9, Fibronectin, Fn1
Background: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling (By similarity).

基因ID

14268

UniProt

P11276