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Cyclin D1 抗体 (AA 61-110)

Name: Cyclin D1 抗体 (AA 61-110)
SKU: ABIN671016
Availability: OutOfStock

This anti-Cyclin D1 antibody is a 兔多克隆 antibody detecting Cyclin D1 in WB, ELISA, FACS, IHC (p), IF (cc), IF (p) 和 IHC (fro). Suitable for 人, 小鼠, 大鼠, Cow, 兔和绵羊. This Primary Antibody has been cited in 15+ publications.

A549 cells probed with Anti-Cyclin D1 Polyclonal Antibody at 1:5000 90min in 37˚C. (Cyclin D1 抗体 (AA 61-110))

Formalin-fixed and paraffin embedded human placenta tissue labeled with Anti-Cyclin D1 Polyclonal Antibody, Unconjugated (ABIN671016) at 1:200, followed by conjugation to the secondary antibody and DAB staining (Cyclin D1 抗体 (AA 61-110))

IHC

RSC96 cells probed with Cyclin D1 Polyclonal Antibody, Unconjugated at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange). (Cyclin D1 抗体 (AA 61-110))

FACS

Image provided by One World Lab validation program. (Cyclin D1 抗体 (AA 61-110))

IF (cc)

6 images

(15 references)

产品编号 ABIN671016

发货至: 中国

Quick Overview for Cyclin D1 抗体 (AA 61-110) (ABIN671016)

抗原

See all Cyclin D1 (CCND1) 抗体

Cyclin D1 (CCND1)

适用

人, 小鼠, 大鼠, Cow, 兔, 绵羊

宿主

兔

克隆类型

多克隆

标记

This Cyclin D1 antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

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AA 61-110

交叉反应

Cow, 人, 小鼠, 兔, 大鼠, 绵羊

预测反应

Dog

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from mouse Cyclin D1

亚型

IgG
anti-Cyclin D1 (CCND1) (C-Term) antibody
CCND1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Cyclin D1 (CCND1) antibody
KO Validated CCND1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated

anti-Cyclin D1 (CCND1) (AA 167-295) antibody
CCND1 适用: 人 IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 3B6G3 unconjugated

anti-Cyclin D1 (CCND1) (AA 1-295) antibody
CCND1 适用: 人, 小鼠 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 1F7F5 unconjugated

anti-Cyclin D1 (CCND1) (C-Term) antibody
CCND1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cyclin D1 (CCND1) (AA 68-97) antibody
CCND1 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB8066 unconjugated

anti-Cyclin D1 (CCND1) (AA 68-97) antibody
CCND1 适用: 人, 小鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Cyclin D1 (CCND1) antibody
CCND1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal DCS-6 unconjugated

anti-Cyclin D1 (CCND1) (C-Term) antibody
CCND1 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Cyclin D1 (CCND1) (AA 1-233) antibody
CCND1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Jiang, Xie, Cai, Ren, Ma: "Effects of hepatitis C virus core protein and nonstructural protein 4B on the Wnt/β-catenin pathway." in: BMC microbiology, Vol. 17, Issue 1, pp. 124, (2017) (PubMed).

Wang, Cao, Sun, Dai, Fang, Li, Yan, Mao, Zhang: "Total flavonoid aglycones extract in Radix scutellariae inhibits lung carcinoma and lung metastasis by affecting cell cycle and DNA synthesis." in: Journal of ethnopharmacology, Vol. 194, pp. 269-279, (2016) (PubMed).

Zhang, Pan, Xu, Niu, Ma, Xu: "Interleukin 18 augments growth ability via NF-?B and p38/ATF2 pathways by targeting cyclin B1, cyclin B2, cyclin A2, and Bcl-2 in BRL-3A rat liver cells." in: Gene, Vol. 563, Issue 1, pp. 45-51, (2015) (PubMed).

Wang, Yang, Liu, Huang, Wang, Chen, Chen: "RBP-J-interacting and tubulin-associated protein induces apoptosis and cell cycle arrest in human hepatocellular carcinoma by activating the p53-Fbxw7 pathway." in: Biochemical and biophysical research communications, Vol. 454, Issue 1, pp. 71-7, (2015) (PubMed).

Yang, Wang, Hui, Li, Jiang: "SOX 1, contrary to SOX 2, suppresses proliferation, migration, and invasion in human laryngeal squamous cell carcinoma by inhibiting the Wnt/?-catenin pathway." in: Tumour biology, (2015) (PubMed).

Shi, Lu, Zhou, Tan, He, Ding, Tian, Wang, Wang: "Long non-coding RNA Loc554202 regulates proliferation and migration in breast cancer cells." in: Biochemical and biophysical research communications, Vol. 446, Issue 2, pp. 448-53, (2014) (PubMed).

Ye, Li, Li, Yang, Niu, Wang: "Anti-tumor activity and relative mechanism of ethanolic extract of Marsdenia tenacissima (Asclepiadaceae) against human hematologic neoplasm in vitro and in vivo." in: Journal of ethnopharmacology, Vol. 153, Issue 1, pp. 258-67, (2014) (PubMed).

Lin, Wang, Wang, Fang, Zhang, Yang, Guo, Lin, Zhang, Wang: "Lithocarpus polystachyus REHD leaf aqueous extract inhibits human breast cancer growth in vitro and in vivo." in: Nutrition and cancer, Vol. 66, Issue 4, pp. 613-24, (2014) (PubMed).

He, Xiang, Zong, Yan, Yu, Liu, Zou, Yang: "CDK2-AP1 inhibits growth of breast cancer cells by regulating cell cycle and increasing docetaxel sensitivity in vivo and in vitro." in: Cancer cell international, Vol. 14, Issue 1, pp. 130, (2014) (PubMed).

Souza, Silva, Calloway, Kucuk, Rossi, McLemore: "Genistein protects hematopoietic stem cells against G-CSF-induced DNA damage." in: Cancer prevention research (Philadelphia, Pa.), Vol. 7, Issue 5, pp. 534-44, (2014) (PubMed).

Li, Yu, Liu, Zhu, He, Peng, Hua: "miR-34c works downstream of p53 leading to dairy goat male germline stem-cell (mGSCs) apoptosis." in: Cell proliferation, Vol. 46, Issue 2, pp. 223-31, (2013) (PubMed).

Teng, Chang, Wang, Lu, Wu, Yang, Chiu, Yang, Hsu, Ho: "Phospholipid-functionalized mesoporous silica nanocarriers for selective photodynamic therapy of cancer." in: Biomaterials, Vol. 34, Issue 30, pp. 7462-70, (2013) (PubMed).

Wang, Wu, Gao, Chen, Gu, Ding, Guo: "Elevated toll-like receptor 3 inhibits pancreatic ?-cell proliferation through G1 phase cell cycle arrest." in: Molecular and cellular endocrinology, Vol. 377, Issue 1-2, pp. 112-22, (2013) (PubMed).

Zhang, Tan, Wang, Chen, Quan, Xian, Zhang, He, Zhang: "Musashi2 modulates K562 leukemic cell proliferation and apoptosis involving the MAPK pathway." in: Experimental cell research, Vol. 320, Issue 1, pp. 119-27, (2013) (PubMed).

Chen, Shi, Liu, Fang, Zhang, Zhao: "Targeting SPARC by lentivirus-mediated RNA interference inhibits cervical cancer cell growth and metastasis." in: BMC cancer, Vol. 12, pp. 464, (2012) (PubMed).
抗原

Cyclin D1 (CCND1)

别名

Cyclin D1

背景

Synonyms: cD1, CycD1, Cyl-1, PRAD1, bcl-1, AI32739, G1/S-specific cyclin-D1, Ccnd1
Background: Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner (By similarity).

基因ID

12443

UniProt

P25322

途径

PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling