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- 抗原 See all RPH3A 抗体
- RPH3A (Rabphilin 3A (RPH3A))
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抗原表位
- pSer234
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适用
- 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This RPH3A antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC)
- 交叉反应 （详细）
- Cross reactivity with Rab 3A from other species has not been determined, however, the sequence of the immunogen shows 100 % identity to mouse, therefore, cross reactivity is expected.
- 纯化方法
- Anti-Rabphilin 3A pS234 antibody is directed against rat rabphilin 3A protein phosphorylated at serine 234. Rabphilin 3A pS234 antibodies are affinity purified from monospecific antiserum by immunoaffinity purification. The immunolabeling of the rabphilin band was completely blocked by the phosphopeptide used as antigen while the dephosphopeptide had no effect on the immunolabeling.
- 免疫原
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Immunogen: Anti-Rabphilin 3A pS234 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser234 conjugated to KLH.
Immunogen Type: Peptide
- 亚型
- IgG
- Top Product
- Discover our top product RPH3A Primary Antibody
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anti-Rabphilin 3A (RPH3A) (AA 1-280) antibody
RPH3A 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Rabphilin 3A (RPH3A) (pSer234) antibodyRPH3A 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Rabphilin 3A (RPH3A) antibodyRPH3A 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Rabphilin 3A (RPH3A) (pSer234) antibodyRPH3A 适用: 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Rabphilin 3A (RPH3A) (C-Term) antibodyRPH3A 适用: 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Rabphilin 3A (RPH3A) antibodyRPH3A 适用: 人, 小鼠, 大鼠 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Rabphilin 3A (RPH3A) (AA 203-252), (pSer237) antibodyRPH3A 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Rabphilin 3A (RPH3A) antibodyRPH3A 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Rabphilin 3A (RPH3A) (AA 1-280) antibodyRPH3A 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Rabphilin 3A (RPH3A) (Tyr627) antibodyRPH3A 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry Dilution: 1:400
Application Note: Anti-Rabphilin 3A pS234 (Rabbit) antibody is suitable for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 82 kDa in size corresponding to rabphilin 3A protein phosphorylated at Ser234 in the appropriate cell lysate or extract.
Western Blot Dilution: 1:1000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
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- 抗原
- RPH3A (Rabphilin 3A (RPH3A))
- 别名
- Rabphilin 3A (RPH3A 产品)
- 别名
- 2900002P20Rik antibody, AU022689 antibody, AW108370 antibody, CG11556 antibody, Dmel\\CG11556 antibody, exophilin-1 antibody, fc61h10 antibody, rph3a antibody, wu:fc61h10 antibody, rabphilin 3A antibody, Rabphilin antibody, rabphilin-3a, putative antibody, rabphilin-3A antibody, rabphilin 3A S homeolog antibody, rabphilin 3A homolog (mouse), a antibody, RPH3A antibody, Rph3a antibody, Rph antibody, Smp_145120 antibody, Tsp_00335 antibody, rph3a.S antibody, rph3aa antibody
- 背景
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Synonyms: Rabphilin 3A Homolog, Rabphilin-3A, RP3A, Rph3A, Exophilin 1, Exophilin-1
Background: Rabphilin-3A is a peripheral membrane protein that binds Ca2+ and phospholipids and is attached to synaptic vesicle membranes. The expression of rabphilin 3A is reduced in an animal model of Huntington's disease. Rabphilin can be phosphorylated at Ser234 by both CAM kinase II and PKA and this phosphorylation has been suggested to regulate neuronal activity during development in a synapse-specific manner. Anti-Rabphilin 3A antibody is ideal for investigators involved in Signal Transduction, Cell Signaling, and Neuroscience research.
Gene Name: RPH3A
- 基因ID
- 171039
- UniProt
- P47709
- 途径
- Synaptic Vesicle Exocytosis
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