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Dynamin 1 抗体 (pSer774)

DNM1 适用: 大鼠 WB, ELISA, IHC, FM 宿主: 绵羊 Polyclonal unconjugated
Rockland
产品编号 ABIN6658244
Supplier Product No.: 612-601-d32
发货至: 中国
  • 抗原 See all Dynamin 1 (DNM1) 抗体
    Dynamin 1 (DNM1)
    抗原表位
    • 32
    • 21
    • 15
    • 5
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer774
    适用
    • 99
    • 43
    • 41
    • 10
    • 9
    • 8
    • 3
    • 2
    • 2
    • 2
    • 1
    大鼠
    宿主
    • 90
    • 10
    • 2
    绵羊
    克隆类型
    • 100
    • 3
    多克隆
    标记
    • 49
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Dynamin 1 antibody is un-conjugated
    应用范围
    • 87
    • 39
    • 39
    • 34
    • 18
    • 14
    • 13
    • 5
    • 5
    • 4
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)
    Supplier Product No.
    612-601-d32
    Supplier
    Rockland
    原理
    Dynamin phospho S774 Antibody
    交叉反应 (详细)
    Anti-Dynamin pS774 antibody is directed against Dynamin.
    纯化方法
    Anti-Dynamin pS774 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.
    免疫原
    Anti-Dynamin pS774 Antibody was produced in sheep by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser774 conjugated to KLH.
    亚型
    IgG
    Top Product
    Discover our top product DNM1 Primary Antibody
  • 应用备注

    ELISA_Dilution: 1:10,000

    Immunohistochemistry_Dilution: 1:1000

    IF_Microscopy_Dilution: 1:1000

    Western_Blot_Dilution: 1:1000

    说明

    Anti-Dynamin pS774 Antibody is tested for use in Western Blotting and ICC and suitable in IHC and ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 95 kDa in size corresponding to dynamin protein phosphorylated at Ser774 in the appropriate cell lysate or extract. Anti-Dynamin pS774 antibody labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Anti-Dynamin pS774 antibody does not cross react with other purified substrates of cdk5 (e.g. amphiphysin and synapsin).

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液

    Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

    Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    Dynamin 1 (DNM1)
    别名
    Dynamin (DNM1 产品)
    背景

    Synonyms: sheep anti-Dynamin pS774 Antibody, Dynamin-1, B-dynamin, D100

    Background: Dynamin Antibody detects Dynamin which is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis. Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo. Phosphorylation of Ser774 by GSK3 has recently been shown to control activity-dependent bulk endocytosis of synaptic vesicles. Anti-Dynamin pS774 Antibody is ideal for investigators involved in Cell Signaling, Cell Motility, Neuroscience and Cancer Research.

    Gene Name: DNM1

    基因ID
    140694
    UniProt
    P21575
    途径
    Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
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