Dynamin 1 抗体 (pSer774)
Quick Overview for Dynamin 1 抗体 (pSer774) (ABIN6658244)
抗原
See all Dynamin 1 (DNM1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer774
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Supplier Product No.
- 612-601-d32
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Supplier
- Rockland
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原理
- Dynamin phospho S774 Antibody
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交叉反应 （详细）
- Anti-Dynamin pS774 antibody is directed against Dynamin.
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纯化方法
- Anti-Dynamin pS774 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.
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免疫原
- Anti-Dynamin pS774 Antibody was produced in sheep by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser774 conjugated to KLH.
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亚型
- IgG
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anti-Dynamin 1 (DNM1) antibody
DNM1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Dynamin 1 (DNM1) (AA 616-667) antibodyDNM1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Dynamin 1 (DNM1) (AA 572-745) antibodyDNM1 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Dynamin 1 (DNM1) (AA 2-245) antibodyDNM1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Dynamin 1 (DNM1) antibodyDNM1 适用: 人 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 3G4B6 unconjugated
anti-Dynamin 1 (DNM1) antibodyDNM1 适用: 人, 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Dynamin 1 (DNM1) (Ser307) antibodyDNM1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Dynamin 1 (DNM1) (C-Term) antibodyDNM1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Dynamin 1 (DNM1) (pSer778) antibodyDNM1 适用: 大鼠 WB 宿主: 绵羊 Polyclonal unconjugated
anti-Dynamin 1 (DNM1) (N-Term) antibodyDNM1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA_Dilution: 1:10,000
Immunohistochemistry_Dilution: 1:1000
IF_Microscopy_Dilution: 1:1000
Western_Blot_Dilution: 1:1000
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说明
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Anti-Dynamin pS774 Antibody is tested for use in Western Blotting and ICC and suitable in IHC and ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 95 kDa in size corresponding to dynamin protein phosphorylated at Ser774 in the appropriate cell lysate or extract. Anti-Dynamin pS774 antibody labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Anti-Dynamin pS774 antibody does not cross react with other purified substrates of cdk5 (e.g. amphiphysin and synapsin).
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
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有效期
- 12 months
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- Dynamin 1 (DNM1)
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别名
- Dynamin
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背景
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Synonyms: sheep anti-Dynamin pS774 Antibody, Dynamin-1, B-dynamin, D100
Background: Dynamin Antibody detects Dynamin which is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis. Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo. Phosphorylation of Ser774 by GSK3 has recently been shown to control activity-dependent bulk endocytosis of synaptic vesicles. Anti-Dynamin pS774 Antibody is ideal for investigators involved in Cell Signaling, Cell Motility, Neuroscience and Cancer Research.
Gene Name: DNM1
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基因ID
- 140694
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UniProt
- P21575
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途径
- Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
抗原
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