Dynamin 1 抗体 (pSer774)
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- 抗原 See all Dynamin 1 (DNM1) 抗体
- Dynamin 1 (DNM1)
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抗原表位
- pSer774
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适用
- 大鼠
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宿主
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绵羊
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克隆类型
- 多克隆
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标记
- This Dynamin 1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)
- Supplier Product No.
- 612-601-d32
- Supplier
- Rockland
- 原理
- Dynamin phospho S774 Antibody
- 交叉反应 (详细)
- Anti-Dynamin pS774 antibody is directed against Dynamin.
- 纯化方法
- Anti-Dynamin pS774 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.
- 免疫原
- Anti-Dynamin pS774 Antibody was produced in sheep by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser774 conjugated to KLH.
- 亚型
- IgG
- Top Product
- Discover our top product DNM1 Primary Antibody
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- 应用备注
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ELISA_Dilution: 1:10,000
Immunohistochemistry_Dilution: 1:1000
IF_Microscopy_Dilution: 1:1000
Western_Blot_Dilution: 1:1000
- 说明
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Anti-Dynamin pS774 Antibody is tested for use in Western Blotting and ICC and suitable in IHC and ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 95 kDa in size corresponding to dynamin protein phosphorylated at Ser774 in the appropriate cell lysate or extract. Anti-Dynamin pS774 antibody labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Anti-Dynamin pS774 antibody does not cross react with other purified substrates of cdk5 (e.g. amphiphysin and synapsin).
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Dynamin 1 (DNM1)
- 别名
- Dynamin (DNM1 产品)
- 背景
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Synonyms: sheep anti-Dynamin pS774 Antibody, Dynamin-1, B-dynamin, D100
Background: Dynamin Antibody detects Dynamin which is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis. Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo. Phosphorylation of Ser774 by GSK3 has recently been shown to control activity-dependent bulk endocytosis of synaptic vesicles. Anti-Dynamin pS774 Antibody is ideal for investigators involved in Cell Signaling, Cell Motility, Neuroscience and Cancer Research.
Gene Name: DNM1
- 基因ID
- 140694
- UniProt
- P21575
- 途径
- Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
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