AQP2 抗体 (pSer261)
Quick Overview for AQP2 抗体 (pSer261) (ABIN6658216)
抗原
See all AQP2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer261
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Supplier Product No.
- 612-401-d08
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Supplier
- Rockland
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原理
- Aquaporin 2 phospho S261 Antibody
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交叉反应 （详细）
- Anti-Aquaporin 2 pS261 Antibody is directed against rat Aquaporin 2 protein phosphorylated at S261.
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纯化方法
- The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.
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免疫原
- Anti-Aquaporin 2 pS261 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser261.
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亚型
- IgG
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anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 171-271) antibody
AQP2 适用: 人, 大鼠, 小鼠 WB, ELISA, IF (p), IF (cc), IHC (p), ICC, FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 177-271) antibodyAQP2 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 147-271) antibodyAQP2 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 149-271) antibodyAQP2 适用: 人 ELISA 宿主: 小鼠 Monoclonal 7H8B5 unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 241-271), (C-Term) antibodyAQP2 适用: 人, 大鼠, 小鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 245-260) antibodyAQP2 适用: 人, 大鼠, 小鼠 WB, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (C-Term) antibodyAQP2 适用: 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 101-200) antibodyAQP2 适用: 人, 大鼠, 小鼠 WB, ELISA, IF (p), IF (cc), IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (pSer269) antibodyAQP2 适用: 大鼠 WB, ELISA, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (C-Term) antibody (HRP)AQP2 适用: 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal HRP
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应用备注
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ELISA_Dilution: 1:10,000
Western_Blot_Dilution: 1:1000
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说明
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Anti-Aquaporin 2 pS261 antibody is tested for use in ICC, IHC, IP, and WB. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 29 kDa AQP2 protein phosphorylated at Ser261 in the appropriate cell lysate or extract. This antibody also recognizes the glycosylated for of aquaporin 2 at approximately 37 kDa in size.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
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有效期
- 12 months
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- AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))
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别名
- Aquaporin 2
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背景
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Synonyms: ADH water channel, Aquaporin-CD, Collecting duct water channel protein, WCH-CD, Water channel protein for renal collecting duct
Background: Aquaporin 2 pS261 antibody recognizes aquaporin 2 (AQP2) which is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans. A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells. Recently, serine-261 has been identified as a novel phosphorylation site on AQP2 and levels of phosphorylated S261 have been shown to decrease with vasopressin treatment suggesting its involvement in vasopressin-dependent AQP2 trafficking. Anti-Aquaporin 2 pS261 antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.
Gene Name: AQP2
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基因ID
- 25386
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UniProt
- P34080
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途径
- Response to Water Deprivation
抗原
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