MARCKS 抗体 (pSer152, pSer156)
Quick Overview for MARCKS 抗体 (pSer152, pSer156) (ABIN6658212)
抗原
See all MARCKS 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer152, pSer156
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Supplier Product No.
- 612-401-d70
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Supplier
- Rockland
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原理
- MARCKS phospho S152/phospho S156 Antibody
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交叉反应 (详细)
- MARCKS antibody are affinity purified from monospecific antiserum by immunoaffinity purification.
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纯化方法
- Anti-MARCKS pS152/pS156 antibody is directed against MARKS doubly phosphorylated at Ser152 and Ser156.
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免疫原
- Anti-MARCKS pS152/pS156 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser 152/156 conjugated to KLH.
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亚型
- IgG
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应用备注
- Optional[Neutralization_Dilution]: 1:1000
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说明
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Anti-MARCKS pS152/pS156 (Rabbit) antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 87 kDa in size corresponding to MARCKS protein phosphorylated at Ser 152/156 in the appropriate cell lysate or extract.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
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有效期
- 12 months
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- MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))
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别名
- MARCKS
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背景
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Synonyms: Myristoylated alanine-rich C-kinase substrate, Protein kinase C substrate 80 kDa protein
Background: MARCKS pS152/pS156 antibody detects MARKS when phosphoylated at both Ser152 and Ser156 Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC). The phosphorylation of Ser152/156 can be used as a measure of PKC activation although these sites are also phosphorylated by PRK1 MARCKS is a member of a family of calmodulin binding proteins and phosphorylation of Ser 152/156 modulates the binding of MARCKS to calmodulin. Therefore, phosphorylated MARCKS antibody is ideal for investigators involved in Cell Signaling, Cell migration and Cancer Research.
Gene Name: MARCKS
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基因ID
- 3028
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UniProt
- P30009
抗原
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